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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01321
  • pan locus tag?: SAUPAN003701000
  • symbol: SAOUHSC_01321
  • pan gene symbol?: thrC
  • synonym:
  • product: threonine synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01321
  • symbol: SAOUHSC_01321
  • product: threonine synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1264705..1265766
  • length: 1062
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGAAGATGGCAAGGATTAGTAGAAGAGTTTAAAGCACATTTACCAGTAAATGAAAAT
    ACACCAAAATTAACATTGAACGAGGGAAATACACCACTCATTCATTGTGAAAATATGTCT
    AAAATACTAGGCATAGATTTATATGTGAAGTATGAAGGTGCCAATCCGACAGGTTCATTT
    AAAGATCGCGGTATGGTAATGGCTGTGACAAAAGCAAAAGAGCAAGGTAAGAAAATTGTA
    ATATGCGCTTCGACTGGAAATACATCAGCGTCTGCAGCAGCATATGCAGCGAGAGCAGGT
    TTAAAAGCTATCGTCGTAATACCAGAAGGTAAAATTGCATTAGGTAAATTGTCGCAAGCA
    GTAATGTATGGTGCAGAAATCGTTTCTATTGAAGGAAACTTTGATGAAGCTTTAGAAATT
    GTAAAAGAAATTGCAAAAAGTGGCGAAATCGAGCTTGTAAACTCTGTCAATCCATTTAGA
    ATCGAAGGACAAAAGACAGGCTCATTTGAAATTGTACAACAATTAGACGGTGAAGCACCT
    GATATTTTAGCGATTCCTGTAGGTAATGCAGGTAATATTACTGCATATTGGAAAGGCTTT
    AAAGAATATCATGAAGCTAAAGGATCACAATTGCCGAAAATGTTTGGCTTCCAAGCTGAA
    GGCGCATCACCAATTGTTCAAAATAAAGTCATTAAAAATCCTGAAACGATTGCAACTGCT
    ATTCGAATTGGTAATCCTGCTAGTTGGGATAAGGCGACTAATGCTCTTAAAGAATCAAAT
    GGATTAATAGATAGTGTTACTGATGATGAAATTCTAGAAGCATATCAGTTAATGACAACT
    AAAGAAGGTGTCTTTAGTGAACCAGCGAGTAATGCTTCTATTGCAGGTTTAATTAAATTG
    CATAGACAAGGTAAATTACCTCAAGGTAAAAAAGTAGTTGCTATTTTAACTGGTAATGGA
    TTAAAAGATCCTGATACTGCTATTTCACTACTAGATAATCCGATAAAGCCATTGCCAAAT
    GATAAAGATAGCATTATCGATTATATTAAAGGAGCTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01321
  • symbol: SAOUHSC_01321
  • description: threonine synthase
  • length: 353
  • theoretical pI: 6.1777
  • theoretical MW: 37869.2
  • GRAVY: -0.172805

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.3.1?  ExPASy
    Threonine synthase O-phospho-L-homoserine + H2O = L-threonine + phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 352.3)
    and 17 more
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 105.6)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 85.8)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 78.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
    Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 78.7)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 77)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 72.7)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 69.4)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 60.3)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 54.2)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 52.3)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 48.5)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 36.8)
    Metabolism Central intermediary metabolism Other 1-aminocyclopropane-1-carboxylate deaminase (TIGR01274; EC 3.5.99.7; HMM-score: 29.8)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 26.1)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 23)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 20.1)
  • TheSEED  :
    • Threonine synthase (EC 4.2.3.1)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Threonine synthase (EC 4.2.3.1)
  • PFAM:
    no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 263)
    and 1 more
    PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008553
    • TAT(Tat/SPI): 0.000483
    • LIPO(Sec/SPII): 0.000737
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILGIDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLKAIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFRIEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQAEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQLMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNPIKPLPNDKDSIIDYIKGAL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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