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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01911
  • pan locus tag?: SAUPAN004505000
  • symbol: SAOUHSC_01911
  • pan gene symbol?: tnp2
  • synonym:
  • product: IS200 family transposase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01911
  • symbol: SAOUHSC_01911
  • product: IS200 family transposase
  • replicon: chromosome
  • strand: -
  • coordinates: 1820862..1821347
  • length: 486
  • essential: no [1] DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTCATCTGACACAAACAGTTTAGCACATACAAAATGGAATTGTAAGTATCATATTGTC
    TTTGCACCTAAATACAGAAGACAAGTGATATATGGAAAAATAAAAAAAGATATAGGGATT
    ATATTGCGTCAATTATGTGAAAGAAAAGGTGTAGAGATAATTGAAGCAGAGGCATGTAAA
    GATCATATCCATATGTTAGTTAGTATACCACCCAAACTTGGGGTATCATCATTTGTTGGC
    TATTTAAAAGGAAAAAGTAGTTTAATGATATTTGATAGACATGCTAACTTAAAGTATAGA
    TATGGAAATAGAAAGTTTTGGTGTAAAGGATTTTATGTGGATACAGTAGGTAGAAATAAA
    AAAGTGATTGAAAATTATATTCGTAATCAATTACAAGAGGATATCGTTGCAGACCAAATC
    TCAATGGAAGAATATTTAGATCCTTTCACTGGAGAGAAAAATAAAAAAAGAAAGAAAAAA
    GAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    486

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01911
  • symbol: SAOUHSC_01911
  • description: IS200 family transposase
  • length: 161
  • theoretical pI: 10.1718
  • theoretical MW: 18905.9
  • GRAVY: -0.599379

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01109138: hypothetical protein
  • PFAM:
    HUH (CL0481) Y1_Tnp; Transposase IS200 like (PF01797; HMM-score: 129.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003054
    • TAT(Tat/SPI): 0.00031
    • LIPO(Sec/SPII): 0.001118
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSSDTNSLAHTKWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQLCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYGNRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKKE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]