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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01991
  • pan locus tag?: SAUPAN004794000
  • symbol: SAOUHSC_01991
  • pan gene symbol?: glnP
  • synonym:
  • product: ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01991
  • symbol: SAOUHSC_01991
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1894060..1895517
  • length: 1458
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGTGTTTAATTAGGTTTATATTAGTATTAGGGTTGTTAATTAGCAGTGCTATGGTG
    TATATCAATCCCACAGCACATGCTGAACAAGATCAAACATGGGAGAAGATTAAAGAACGC
    GGTGAACTTAGAGTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTT
    AATGGTAAGACTGAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGAT
    AATAATTTAAAATTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAA
    ACTGGAAAAATTGATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAA
    GTTGATTTTTCAGATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAA
    GTTAATGAATATAAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGA
    ACTGAACAAGAAAAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGC
    CGTTTACCAGACGTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAA
    AAACCTGTAGCAGAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAA
    TTTAATGAAGAAGAAAAAGATACAGTGATAGCAGTGCCAAAAGATTCACCAAAATTATTG
    TCACAAATTAATAAAACGATTAAGGAGGTTAAAGATAAAGGATTAATCGATAAATATATG
    ACTAATGCTGCAAATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTT
    TTCTTGAAAGGAATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCT
    ATTTTAGGTGCATTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATT
    GCTTCTATCTATATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTA
    TTCTTTGGTATAACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATT
    GCATTAGTTATTAATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCT
    GTTGATAAAGGCCAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATG
    AAAAGTGTAATTATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTT
    GTCACTTTAATTAAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTT
    AATGCACAAGTGGTTCAAGGTATTTCATTTGACCCATTCACACCATTAATAGTGGCAGCA
    GCATTATACTTTGTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGA
    TTGAATGCCAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01991
  • symbol: SAOUHSC_01991
  • description: ABC transporter permease
  • length: 485
  • theoretical pI: 9.56041
  • theoretical MW: 53122.2
  • GRAVY: 0.212371

⊟Function[edit | edit source]

  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 159)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 136.6)
    and 12 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 59.7)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.9)
    extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 41.1)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 29.8)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.7)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.2)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR02285; HMM-score: 14.5)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 11.9)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 11.6)
  • TheSEED  :
    • Arginine ABC transporter, substrate-binding and permease protein
  • PFAM:
    PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 190.2)
    and 3 more
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 85.5)
    PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 37.8)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 16.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.995819
    • TAT(Tat/SPI): 0.001914
    • LIPO(Sec/SPII): 0.001537
    • Cleavage Site: CS pos: 28-29. AHA-EQ. Pr: 0.9908
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELRVGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, ArgR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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