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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02368
  • pan locus tag?: SAUPAN005421000
  • symbol: pyrG
  • pan gene symbol?: pyrG
  • synonym:
  • product: CTP synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02368
  • symbol: pyrG
  • product: CTP synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 2190059..2191669
  • length: 1611
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACAAAATTTATTTTTGTAACAGGTGGCGTAGTTTCATCATTAGGGAAGGGTATTACA
    GCATCTTCTCTAGGTAGATTATTAAAAGATAGAGGTCTAAATGTAACAATTCAAAAATTC
    GATCCATACTTAAATGTTGACCCAGGTACAATGAGTCCTTATCAACATGGTGAAGTATTC
    GTAACGGATGATGGTGCAGAAACTGACCTAGACTTAGGACATTACGAAAGATTTATTGAT
    ATTAATTTAAACAAGTTTTCAAATGTGACAGCCGGTAAAGTGTATTCACACGTATTGAAA
    AAAGAACGTCGTGGTGATTACTTAGGCGGAACAGTTCAAGTTATTCCGCATATTACAAAT
    GAAATTAAAGAACGTTTATTACTTGCAGGGGAAAGTACGAATGCAGACGTTGTTATCACT
    GAAATTGGCGGTACAACAGGTGATATTGAGTCATTACCGTTTATTGAAGCGATTCGTCAA
    ATTCGTAGCGATTTAGGTAGAGAAAATGTTATGTATGTTCACTGTACATTACTGCCTTAT
    ATTAAAGCTGCTGGAGAAATGAAAACGAAGCCAACACAACATAGTGTTAAAGAATTACGA
    GGCTTAGGTATTCAACCAGACTTAATCGTTGTAAGAACTGAATATGAAATGACACAAGAT
    TTAAAAGATAAAATTGCATTATTCTGTGACATTAATAAAGAAAGTGTTATTGAATGTCGT
    GATGCAGACTCTTTATACGAAATTCCATTACAATTAAGCCAACAAAATATGGATGATATC
    GTTATTAAACGTTTACAATTAAACGCGAAATATGAAACACAGCTTGATGAATGGAAACAG
    TTGTTAGATATCGTTAATAATTTAGATGGTAAAATTACAATTGGTTTAGTAGGTAAATAT
    GTTAGCTTACAAGATGCATATTTATCAGTTGTTGAATCATTGAAACATGCTGGATATCCT
    TTTGCCAAAGATATTGACATTAGATGGATTGATTCAAGTGAAGTAACAGATGAAAATGCA
    GCCGAATACCTTGCAGATGTCGACGGTATTTTAGTACCAGGTGGATTTGGTTTCCGTGCA
    AGTGAAGGTAAAATTAGTGCAATTAAGTATGCTAGAGAAAACAATGTACCATTCTTTGGT
    ATTTGTTTAGGAATGCAACTTGCAACAGTTGAATTTTCAAGAAACGTATTAGGCCTTGAA
    GGCGCACATTCAGCTGAATTAGACCCAGCAACACCATACCCAATTATAGATTTATTACCA
    GAACAAAAAGATATCGAAGATTTAGGTGGTACATTACGCTTAGGCTTATATTCATGTTCA
    ATTAAAGAAGGCACATTGGCACAAGATGTTTATGGTAAAGCGGAAATTGAAGAAAGACAT
    CGTCATCGTTATGAATTTAATAATGACTATAGAGAACAATTAGAAGCAAATGGTATGGTG
    ATTTCTGGTACAAGTCCAGATGGACGTTTAGTAGAAATGGTAGAGATTCCGACAAATGAT
    TTCTTTATTGCTTGTCAATTCCACCCAGAATTCTTATCTAGACCAAATCGTCCGCACCCG
    ATTTTTAAATCATTTATTGAAGCTTCATTAAAATATCAACAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1611

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02368
  • symbol: PyrG
  • description: CTP synthetase
  • length: 536
  • theoretical pI: 4.75985
  • theoretical MW: 59981.7
  • GRAVY: -0.257276

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.2?  ExPASy
    CTP synthase (glutamine hydrolyzing) ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 849.4)
    and 5 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 31.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 25.4)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 23)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 16)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 9.7)
  • TheSEED  :
    • CTP synthase (EC 6.3.4.2)
    Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  CTP synthase (EC 6.3.4.2)
    and 1 more
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  CTP synthase (EC 6.3.4.2)
  • PFAM:
    P-loop_NTPase (CL0023) CTP_synth_N; CTP synthase N-terminus (PF06418; HMM-score: 425.4)
    and 5 more
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 188.5)
    Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 41.7)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 13.8)
    GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 12.9)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.107719
    • TAT(Tat/SPI): 0.010687
    • LIPO(Sec/SPII): 0.063485
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKERRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQIRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMTQDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLDEWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSEVTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEFSRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYSCSIKEGTLAQDVYGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFHPEFLSRPNRPHPIFKSFIEASLKYQQNK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02492(rplO)50S ribosomal protein L15  [4] (data from MRSA252)
    SAOUHSC_01757(rplU)50S ribosomal protein L21  [4] (data from MRSA252)
    SAOUHSC_01232(rpsB)30S ribosomal protein S2  [4] (data from MRSA252)
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [4] (data from MRSA252)
    SAOUHSC_01041pyruvate dehydrogenase complex, E1 component subunit beta  [4] (data from MRSA252)
    SAOUHSC_01042branched-chain alpha-keto acid dehydrogenase subunit E2  [4] (data from MRSA252)
    SAOUHSC_01043dihydrolipoamide dehydrogenase  [4] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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