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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02423
  • pan locus tag?: SAUPAN005560000
  • symbol: SAOUHSC_02423
  • pan gene symbol?: —
  • synonym:
  • product: UDP-N-acetylglucosamine pyrophosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02423
  • symbol: SAOUHSC_02423
  • product: UDP-N-acetylglucosamine pyrophosphorylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2250585..2251772
  • length: 1188
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCTAGATAAAAATCAATTAGCTAAATATAAACAAGATCATTTGTGTGAATATGAAAAA
    ATAATGAGTAACAATGAAAAAGAAGCACTTGAAGAGAAGGTAGCGTCATTAGATTTAGAT
    TTTATTGCCAAATTATATAACGATTTATACATCAATAAGAAAACAATAGATGATGTATCT
    GCAGTTTCTGAAGTGAAATATGATATTAAATCACAAATGAGTGATGATGAGATTAAGCGT
    TTAGAGGAACAAGGACTTCAAGCTATTAAAGAAGGACAATTTGCAGTACTTTTGATGGCA
    GGTGGTCAAGGAACAAGACTTGGTTACAAGGGCCCTAAAGGTTCGTTTGAAATTGAAGGT
    GTAAGTTTATTTGAACTTCAAGCTAACCAATTAAAAACATTGAATCATCAATCTGGACAT
    ACGATTCAATGGTATATTATGACAAGTGATATCAATCATGAAGAAACTTTAGCTTATTTC
    GAAGCACATAGTTATTTTGGATATGATCAAGAAGCAATCCATTTCTTTAAACAAGATAAT
    ATTGTGGCGCTTAGTGAAGAAGGGAAGTTAATTTTAAATCAACAGGGTCGTATTATGGAA
    ACGCCGAATGGTAATGGTGGCGTGTTTAAATCTTTAGACAAGGCAGGATACTTAGAAGAA
    ATGTCTAATAATGGCGTTAAGTATATTTTCTTGAATAATATCGACAATGTTTTAGTAAGA
    GTGTTGGATCCACTATTTGCAGGATTTACGGTAGAACATGATTACGATATTACTTCTAAA
    ACGATTCAACCAAAACCAGGAGAAAGTGTTGGACGTTTAGTTAATGTCGATTGTAAAGAT
    ACAGTACTGGAGTATTCAGAATTAGATCCAGAAGTTGCAAATCAATTTAACAATGCTAAT
    ATCGGTATTCATGCATTTAAACTTGGTTTTATTTTAAACGCTGTAAATCGTGAGTTACCT
    TATCATTTAGCTATTAAGAATTTGAAGCAATTAGATGAGAACTTTGGTGTTATTGAACAA
    CCAACTTTAAAATTCGAATTGTTCTATTTTGACATATTTACTTATGGAACTAGTTTTGTC
    ACGTTACAAGTTCCTAGAGAAGAAGAATTTTCACCTCTTAAAAATAAAGAAGGTAAAGAT
    AGTGTTGCAACTGCAACAGAAGATTTACGTAGAATGGGTTTAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02423
  • symbol: SAOUHSC_02423
  • description: UDP-N-acetylglucosamine pyrophosphorylase
  • length: 395
  • theoretical pI: 4.60106
  • theoretical MW: 44893.4
  • GRAVY: -0.391899

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.23?  ExPASy
    UDP-N-acetylglucosamine diphosphorylase UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 17)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 13)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 11.2)
  • TheSEED  :
    • N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23)
  • PFAM:
    GT-A (CL0110) UDPGP; UTP--glucose-1-phosphate uridylyltransferase (PF01704; HMM-score: 141.1)
    and 1 more
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001485
    • TAT(Tat/SPI): 0.000152
    • LIPO(Sec/SPII): 0.000474
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAKLYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGGQGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYFEAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYLEEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNVDCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDENFGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRMGLI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_01801isocitrate dehydrogenase  [3] (data from MRSA252)
    SAOUHSC_02406hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_02407hypothetical protein  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: LexA* (repression) regulon, CcpA* regulon
    LexA*(TF)important in SOS response; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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