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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02760
  • pan locus tag?: SAUPAN006018000
  • symbol: SAOUHSC_02760
  • pan gene symbol?:
  • synonym:
  • product: glutamate synthase subunit alpha

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02760
  • symbol: SAOUHSC_02760
  • product: glutamate synthase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2535539..2537116
  • length: 1578
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACGTTTCTTACAGTCATGCAATTTATAGTTAACATTATCGTTGTAGGATTCATGCTT
    ACGGTTATTGTTATCGGGCTTATTTGGTTAATTAAAGATAAAAGACAATCACAACATAGT
    GTATTAAGGAATTATCCTTTACTAGCACGTATTAGATATATTTCAGAAAAAATGGGACCG
    GAATTACGTCAGTATTTATTTTCTGGGGATAATGAAGGGAAACCTTTTTCACGTAATGAT
    TATAAAAATATCGTTTTGGCTGGAAAATATAACTCTCGTATGACCAGCTTCGGTACTACT
    AAAGATTATCAAGACGGCTTTTACATACAGAACACAATGTTTCCGATGCAACGTAATGAG
    ATTTCAGTAGATAATACAACATTGTTATCAACATTCATTTATAAAATCGCGAATGAGCGT
    TTATTTAGTCGTGAAGAATATCGTGTGCCGACAAAGATTGATCCGTATTACTTAAGTGAT
    GACCATGCAATAAAATTAGGTGAACATTTAAAACATCCATTTATTTTAAAACGTATCGTA
    GGACAATCTGGTATGAGTTATGGCGCTTTAGGAAAAAATGCCATTACAGCTTTATCTAAA
    GGTCTAGCTAAAGCGGGCACTTGGATGAATACAGGTGAAGGTGGCTTATCAGAATATCAT
    TTAAAAGGTAATGGGGATATCATTTTCCAAATTGGTCCCGGTTTATTTGGTGTTCGTGAT
    AAAGAAGGTAATTTTAGTGAAGGTTTATTTAAAGAGGTTGCACAGTTATCTAACGTACGC
    GCATTTGAGCTGAAGTTGGCACAAGGTGCTAAGACTCGTGGTGGTCATATGGAAGCTGAA
    AAGGTAAACGAAGAAATTGCTAAAATCCGAAATGTTGAACCTTATAAAACAATCAATTCA
    CCTAACCGTTACGAATTTATTCATAATGCTGAAGATTTGATTCGTTTCGTCGATCAGTTG
    CAGCAATTAGGTCAAAAACCAGTAGGATTCAAAATTGTAGTAAGCAAAGTTTCAGAAATT
    GAAACACTTGTACGTACGATGGTGGAACTAGATAAGTATCCAAGCTTTATTACGATTGAT
    GGTGGTGAAGGTGGTACTGGTGCAACATTCCAAGAATTACAAGATGGTGTTGGCTTACCG
    CTATTTACAGCTCTACCTATTGTGTCTGGCATGTTAGAAAAATATGGTATTCGAGATAAA
    GTGAAATTGGCGGCATCTGGTAAGTTAGTGACACCAGATAAAATTGCGATTGCACTAGGT
    TTAGGTGCAGATTTTGTAAATATCGCACGTGGGATGATGATTAGTGTCGGTTGTATAATG
    AGTCAACAATGTCACATGAATACGTGTCCTGTAGGTGTTGCAACGACAGATGCGAAGAAA
    GAAAAAGCATTGATTGTTGGAGAAAAGCAATATCGTGTCACAAACTATGTAACAAGTTTG
    CATGAAGGCTTATTCAATATTGCAGCAGCTGTTGGCGTATCCAGTCCTACAGAAATTACT
    GCTGATCATATTGTATATCGAAAAGTCGATGGTGAGTTACAAACGATACATGATTATAAA
    TTAAAACTCATTAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02760
  • symbol: SAOUHSC_02760
  • description: glutamate synthase subunit alpha
  • length: 525
  • theoretical pI: 9.24714
  • theoretical MW: 58439.1
  • GRAVY: -0.122857

Function[edit | edit source]

  • reaction:
    EC 1.4.1.13?  ExPASy
    Glutamate synthase (NADPH) 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH
  • TIGRFAM:
    PfaD family protein (TIGR02814; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.9)
  • TheSEED  :
    • Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
    and 1 more
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
  • PFAM:
    TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 332.5)
    and 2 more
    Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 18.7)
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 15.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002678
    • TAT(Tat/SPI): 0.000389
    • LIPO(Sec/SPII): 0.001608
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRNYPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKDYQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSDDHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSEYHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGHMEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVVSKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGMLEKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCPVGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRKVDGELQTIHDYKLKLIS

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]