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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02771
  • pan locus tag?: SAUPAN006033000
  • symbol: SAOUHSC_02771
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02771
  • symbol: SAOUHSC_02771
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2548409..2548774
  • length: 366
  • essential: no DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGTTAAAAGAAAAAGAAAGTTTTAGATTGCTATATCAAGCTATAAGAGAGATTGCAGAT
    AAAATTGGAGATAATCAGTTAGAAACTAATTCCGTTAGTTTATTATTATTGGACTTTGAT
    TTTGAACATGAAGTATTTGATGAATTGTATCTTGTGATTTTAAAATATTTAAATACAGTA
    AGTATAGAGAACATAAGTCATAGTGAGCTTTTGAATTTAATAGAAAATACGATTCCAGAA
    GATCGAGAGATTAACACGTTTGTAAAAAATAAAATAATAATTGGCTTTGCTAATAATTAC
    TTCCCTGAATTACAAGTATTAGCAAACGAAATAAAATCTGATATGGCTAGTTCATTAAAA
    CAATGA
    60
    120
    180
    240
    300
    360
    366

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02771
  • symbol: SAOUHSC_02771
  • description: hypothetical protein
  • length: 121
  • theoretical pI: 4.2617
  • theoretical MW: 14067
  • GRAVY: -0.0975207

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108624: hypothetical protein
  • PFAM:

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002342
    • TAT(Tat/SPI): 0.000085
    • LIPO(Sec/SPII): 0.000202
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHEVFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFPELQVLANEIKSDMASSLKQ

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [2] [1]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]