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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02967
  • pan locus tag?: SAUPAN006362000
  • symbol: SAOUHSC_02967
  • pan gene symbol?: arcD
  • synonym:
  • product: arginine/ornithine antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02967
  • symbol: SAOUHSC_02967
  • product: arginine/ornithine antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2727712..2729142
  • length: 1431
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACGAATCAGGAGATAACAAACTCAGTAAATCTTCTTTAATTGGACTAGTTATAGGA
    TCCATGATTGGTGGCGGTGCGTTCAATATAATGTCTGATATGGGCGGTAAAGCCGGTGGA
    TTAGCCATTATTATTGGTTGGATTATTACAGCTATAGGAATGATTTCATTAGCGTTCGTA
    TTTCAAAATTTAACCAATGAACGGCCGGAGCTAGACGGTGGTATTTATAGTTATGCTCAA
    GCAGGATTTGGCGATTTTGTAGGATTTATCAGTGCTTGGGGATATTGGTTCTCAGCGTTT
    TTAGGCAATGTTGCCTATGCAACACTATTGATGTCAGCAGTAGGTAACTTTTTCCCGATT
    TTTAAAGGAGGCAACACATTACCAAGTGTTATTGTCGCCTCGTTACTACTCTGGGGTGTC
    CATTTCTTGATTTTAAAAGGCGTTGAAACAGCAGCATTTATCAATAGTATTGTTACTGTT
    GCAAAGTTAATACCGATTTTACTTGTAATCATATGCATGATAATTGCATTCAATTTTGAC
    ACTTTTAAAACAGGCTTTTTCAGTATGACGTCAGAGGGTGTATTGCCATTTAGTTGGGCG
    AGCACAATGAGCCAAGTTAAAAGTACGATGCTAGTGACAGTTTGGGTGTTTATCGGTATC
    GAAGGTGCAGTAATTTTTTCTAGTAGAGCTAAAAATAAAAAAGATGTAGGTAGTGCCACG
    GTTATAGGACTTATATCAGTTTTAATTATCTATTTCTTATTAACTGTATTAGCTCAAGGC
    GTGATTTTGCAAAATCATATTTCGCAATTAGATTCGCCAAGTATGGCACAGGTGCTTGCA
    ACTATTGTAGGTGGTTGGGGATCTACACTTGTAAATATTGGTTTAATTATTTCGGTACTA
    GGTGCATGGTTAGGATGGACACTGCTTGCTGGTGAATTACCTTTCATTGTTGCAAAAGAT
    GGATTATTTCCAAAATGGTTTGCTAAAGAAAATAAAAATGGAGCACCTGTAAATGCACTG
    CTTATTACCAATATATTAGTACAATTATTTTTAATAAGTATGCTATTTACACAGAGTGCG
    TATCAATTTGCATTTTCACTAGCATCAAGTGCTATTTTATACCCTTACATGTTCAGTGCA
    TTTTACCAAGTTAAATACACTTTAGAGCATCGACAGCAAGCAACTACTAAACAATGGACG
    ATTGGTATCATAGCCTCAATTTATGCTATATGGCTTATATATGCAGCAGGTATCAATTAC
    TTATTATTGACGATGTTACTTTATATTCCAGCTCTTCTTGTTTATACAATCGTTCAAAAG
    AATAATCAGACACGTTTGATTAAATCAGACTATATTCTTTTTATGATTATTATCGTACTT
    GCAGTTATCGGGTTAATTAAGTTATTGATGGGAACGATAAATGTTTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1431

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02967
  • symbol: SAOUHSC_02967
  • description: arginine/ornithine antiporter
  • length: 476
  • theoretical pI: 9.50412
  • theoretical MW: 51716.2
  • GRAVY: 0.907983

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 619.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 568.9)
    and 8 more
    histidine-histamine antiporter (TIGR04298; HMM-score: 140)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 99)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 81.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 59.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 51.9)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 46.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 24.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines spore germination protein (amino acid permease) (TIGR00912; HMM-score: 21.5)
  • TheSEED  :
    • Arginine/ornithine antiporter ArcD
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine/ornithine antiporter ArcD
    and 2 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine/ornithine antiporter ArcD
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Arginine/ornithine antiporter ArcD
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 240.8)
    and 4 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 84.2)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 30.4)
    no clan defined YwiC; YwiC-like protein (PF14256; HMM-score: 17.9)
    Gemini_mov; Geminivirus putative movement protein (PF01708; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012824
    • TAT(Tat/SPI): 0.000841
    • LIPO(Sec/SPII): 0.020508
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAIIIGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTVAKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFIGIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMAQVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNGAPVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQQATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSDYILFMIIIVLAVIGLIKLLMGTINVF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR* (repression) regulon, CcpA* regulon, Rex* (repression) regulon, ArcR* (activation) regulon
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArcR*(TF)important in Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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