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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
  • pan locus tag?: SAUPAN002623000
  • symbol: SAUSA300_0712
  • pan gene symbol?: dtpT
  • synonym:
  • product: amino acid/peptide transporter (peptide:H+ symporter)

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
  • symbol: SAUSA300_0712
  • product: amino acid/peptide transporter (peptide:H+ symporter)
  • replicon: chromosome
  • strand: -
  • coordinates: 789740..791245
  • length: 1506
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACACAACAAAACTCCCATGGAAATCAAATTCAAGACATACCTCAAACAGGATTTTTC
    GGGCATCCTCGAGGACTAGGCGTACTCTTCTTTGTAGAGTTCTGGGAAAGGTTTAGTTAT
    TATGGCATGCGTGCCCTACTCATTTTCTACATGTACTTTGCCGTAACAGATAATGGCCTT
    GGAATTGATAAAACAACAGCTATGTCAATTATGTCAGTTTATGGTTCATTAATCTATATG
    ACATCAATACCAGGCGGATGGATTGCTGACAGAATTACAGGCACTAGAGGCGCTACTTTA
    TTAGGTGCAGTCTTTATTATTATCGGACATATTTGTTTAAGCTTACCATTTGCATTAATC
    GGCTTATTCACATCAATGTTCTTCATCATTATTGGTTCAGGTTTAATGAAGCCAAACATT
    TCAAATATCGTTGGCCGTTTATATCCTGAAAATGATAGACGTATGGATGCAGGTTTTGTT
    ATTTTCTATATGTCAGTTAATATGGGTGCATTATTATCACCTATTATTTTGCAACACTTT
    GTTAATGTTAAAAACTTCCACGGCGGATTCTTGATTGCAGCAGTTGGTATGGCATTAGGT
    TTAGTATGGTATGTACTTTTCAACCGCAAAAACTTAGGTAGCGTTGGTATGAAACCTACT
    AACCCATTGACACCAGCTGAAAAGAAAAAGTATGGTCTTATTATCGGAAGTGTTGTTTTA
    GCAATTGTATTAATTATCGTTATTGGTGCATTAACTAACTCATTATCATTTAACTTAGTT
    AGTAATACTGTTTTAGTATTAGGTATTGCATTACCAATCATTTACTTCACTTTAATTATT
    AGAAGTAAAGATGTCACTGATACTGAACGTTCTCGTGTTAAAGCATTTATTCCATTATTT
    ATTCTTGGAATGGTGTTCTGGGCTATTCAAGAACAAGGGTCTAATGTATTAAACATATAT
    GGAATTGAACATTCAGATATGAAATTAAACTTATTTGGTTGGAAAACAAACTTTGGTGAA
    GCGATCTTCCAATCAATTAACCCATTATTTATTTTATTACTAGCTCCAATTATTTCACTT
    TTATGGCAAAAGCTTGGAACTAAACAACCTAGCCTGCCAGTAAAATTTGCAATTGGTACG
    TTCTTAGCAGGTGCGTCATACATACTAATTGGTATTGTAGGTTATGCATCAGGTTCATCA
    AACTTTTCAGTTAACTGGGTTATTTTATCGTACATTATTTGTGTTATCGGTGAGCTATGC
    TTATCACCAACGGGTAATAGTGCTGCTGTTAAGTTAGCACCTAAAGCATTTAATGCCCAA
    ATGATGAGTATTTGGTACTTAACTAACGCTTCTGCACAAGCAATCAATGGTACTTTAGTT
    AAGTTAATAGAACCACTAGGTCAAACAAATTACTTTATTTTCCTAGGTGTTGTTGCAATT
    ATTGTTACAACAATTGTATTAGCAATCTCACCTTTAATCATCAAAGCGATGAAAGGTATA
    CGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1506

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0712 [new locus tag: SAUSA300_RS03825 ]
  • symbol: SAUSA300_0712
  • description: amino acid/peptide transporter (peptide:H+ symporter)
  • length: 501
  • theoretical pI: 9.98893
  • theoretical MW: 54793.8
  • GRAVY: 0.731337

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid/peptide transporter (Peptide:H+ symporter) (TIGR00924; HMM-score: 364.8)
    and 1 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide transporter, peptide:H+ symporter (TIGR00926; HMM-score: 70.9)
  • TheSEED  :
    • Di/tripeptide permease YjdL
    Membrane Transport Uni- Sym- and Antiporters Proton-dependent Peptide Transporters  Di/tripeptide permease YjdL
  • PFAM:
    MFS (CL0015) PTR2; POT family (PF00854; HMM-score: 202.6)
    and 1 more
    MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 63.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001554
    • TAT(Tat/SPI): 0.000134
    • LIPO(Sec/SPII): 0.000376
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMRALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLGAVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFVIFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMKPTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYFTLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGWKTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGIVGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNASAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]