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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
  • pan locus tag?: SAUPAN003563000
  • symbol: SAUSA300_1155
  • pan gene symbol?: rasP
  • synonym:
  • product: putative membrane-associated zinc metalloprotease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
  • symbol: SAUSA300_1155
  • product: putative membrane-associated zinc metalloprotease
  • replicon: chromosome
  • strand: +
  • coordinates: 1266324..1267508
  • length: 1185
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGTCCAGAATTTGCGATCGGTATGGGGCCAAAAATTTTTAGTTTTAGAAAAAATGAA
    ACACTTTACACTATTAGGTTATTGCCTGTTGGTGGATATGTTCGTATGGCAGGAGATGGC
    TTAGAAGAGCCACCAGTCGAGCCCGGTATGAACGTTAAAATTAAACTTAATGAAGAAAAT
    GAAATAACACATATCATATTAGATGATCATCATAAGTTTCAACAAATTGAAGCGATCGAA
    GTTAAAAAATGTGATTTTAAGGATGACTTATTCATAGAAGGTATCACTGCTTATGATAAT
    GAAAGACATCATTTTAAAATTGCTAGAAAGTCTTTCTTTGTTGAAAATGGTAGCTTAGTT
    CAAATTGCTCCGAGAGACAGACAATTTGCACATAAAAAGCCATGGCCGAAATTTTTAACA
    TTATTTGCGGGACCGTTATTTAACTTTATATTAGCTTTAGTCCTATTTATTGGTCTTGCA
    TATTATCAAGGCACGCCTACGTCTACTGTAGAACAAGTCGCAGATAAGTATCCAGCTCAA
    CAAGCAGGATTACAAAAAGGTGATAAGATCGTCCAAATTGGCAAATATAAAATATCTGAA
    TTTGATGATGTTGATAAGGCGTTAGATAAAGTTAAAGATAATAAGACGACTGTTAAATTT
    GAACGTGATGGTAAAACAAAGTCAGTTGAATTAACACCTAAAAAGACTGAAAAAAAACTG
    ACTAAAGTAAGTTCAGAGACGAAGTATGTTCTCGGATTCCAACCAGCGAGTGAACATACA
    CTTTTTAAACCAATTGTATTCGGATTTAAAAGCTTTTTAATCGGTAGTACTTATATTTTT
    ACAGCTGTAGTAGGTATGTTGGCTAGTATATTTACGGGCGGATTCTCATTTGATATGTTA
    AATGGTCCGGTTGGTATTTATCATAACGTCGACTCAGTTGTTAAAGCGGGTATCATTAGC
    TTAATTGGTTACACTGCGTTATTAAGTGTAAACTTAGGTATTATGAATTTAATTCCTATT
    CCTGCACTAGACGGTGGTCGTATTTTATTTGTTATATATGAAGCGATTTTCAGAAAACCA
    GTTAATAAAAAAGCGGAAACAACGATTATTGCTATTGGTGCCATTTTCATGGTCGTTATA
    ATGATATTAGTAACGTGGAATGATATTCGACGATATTTCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
  • symbol: SAUSA300_1155
  • description: putative membrane-associated zinc metalloprotease
  • length: 394
  • theoretical pI: 9.49068
  • theoretical MW: 44279.5
  • GRAVY: 0.0705584

Function[edit | edit source]

  • reaction:
    EC 3.4.24.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 236.6)
    and 7 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
    Cellular processes Cellular processes Sporulation and germination stage IV sporulation protein B (TIGR02860; EC 3.4.21.116; HMM-score: 29.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
    Genetic information processing Protein fate Protein modification and repair C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
    putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 19.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 16)
  • TheSEED  :
    • Membrane-associated zinc metalloprotease
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Membrane-associated zinc metalloprotease
    and 1 more
    Stress Response Periplasmic Stress Periplasmic Stress Response  Membrane-associated zinc metalloprotease
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 207.9)
    and 4 more
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 44.4)
    PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 23.5)
    Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 13.5)
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 10.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004266
    • TAT(Tat/SPI): 0.000439
    • LIPO(Sec/SPII): 0.00085
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MCPEFAIGMGPKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILDDHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRDRQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGLQKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTKVSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDMLNGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFRKPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]