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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
  • pan locus tag?: SAUPAN004913000
  • symbol: SAUSA300_1872
  • pan gene symbol?: gatD
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
  • symbol: SAUSA300_1872
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2032024..2032755
  • length: 732
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCATGAATTGACTATTTATCATTTTATGTCAGATAAATTGAATTTATACAGTGATATA
    GGAAATATTATTGCTTTAAGACAACGTGCTAAAAAACGAAATATTAAAGTTAATGTCGTA
    GAAATCAATGAAACAGAAGGTATTACCTTTGATGAATGTGATATTTTCTTTATCGGTGGT
    GGAAGTGATAGAGAACAAGCATTAGCAACAAAAGAATTAAGTAAAATTAAGACACCACTT
    AAAGAAGCGATTGAAGATGGTATGCCGGGATTAACGATTTGTGGAGGCTATCAATTTTTA
    GGGAAAAAATATATCACGCCTGATGGTACAGAATTAGAAGGGTTAGGTATTTTAGATTTT
    TATACTGAATCAAAGACAAACCGATTAACAGGAGATATTGTTATCGAAAGTGATACTTTT
    GGAACTATTGTAGGTTTTGAAAATCACGGTGGTAGAACATATCATGATTTCGGTACACTT
    GGTCATGTTACTTTTGGTTATGGTAATAATGATGAAGATAAAAAAGAAGGCATTCATTAT
    AAAAATTTATTAGGTACTTATTTACATGGACCAATTTTACCTAAAAATTACGAAATCACT
    GATTATCTGTTAGAAAAAGCTTGTGAACGTAAGGGTATTCCGTTTGAGCCTAAAGAAATA
    GATAATGAAGCGGAAATACAAGCGAAACAAGTATTAATAGACAGAGCAAATAGACAGAAG
    AAATCTCGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    732

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1872 [new locus tag: SAUSA300_RS10240 ]
  • symbol: SAUSA300_1872
  • description: hypothetical protein
  • length: 243
  • theoretical pI: 5.41856
  • theoretical MW: 27429.8
  • GRAVY: -0.530864

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 55)
    and 4 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 19.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyrinic acid a,c-diamide synthase (TIGR00379; EC 6.3.1.-; HMM-score: 19)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 16.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 14.4)
  • TheSEED  :
    • Lipid II isoglutaminyl synthase (glutamine-hydrolyzing), GatD subunit (EC 6.3.5.13)
  • PFAM:
    Glutaminase_I (CL0014) GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 148.6)
    and 3 more
    GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 23.4)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 14.3)
    no clan defined DUF4154; Domain of unknown function (DUF4154) (PF13689; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003013
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.000358
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINETEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGKKYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTLGHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPKEIDNEAEIQAKQVLIDRANRQKKSR

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)