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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2608 [new locus tag: SAUSA300_RS14500 ]
  • pan locus tag?: SAUPAN006430000
  • symbol: hisH
  • pan gene symbol?: hisH
  • synonym:
  • product: imidazole glycerol phosphate synthase subunit HisH

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2608 [new locus tag: SAUSA300_RS14500 ]
  • symbol: hisH
  • product: imidazole glycerol phosphate synthase subunit HisH
  • replicon: chromosome
  • strand: -
  • coordinates: 2835689..2836267
  • length: 579
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAA
    CATTTAGGGTATGAGGTGGTTGTCTCAAATACCTCAAAAATAATCGATCAAGCAGAAACA
    ATCATATTGCCCGGTGTCGGCCATTTTAAAGATGCGATGTCAGAGATAAAACGATTAAAT
    CTCAATGCAATATTGGCTAAGAATACTGATAAGAAGATGATTGGTATTTGTTTAGGCATG
    CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGA
    AATATTTCGCGTATCCAAACAGAATACCCAGTGCCACACTTAGGCTGGAATAATTTAGTG
    AGTAAGCACCCTATGTTAAATCAAGATGTTTATTTCGTACATTCTTATCAAGCGCCGATG
    TCAGAAAATGTAATTGCATATGCGCAGTATGGGGCTGATATTCCGGCAATTGTTCAATTT
    AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATT
    TTGCGTCAGGCAATACAAGGGGGATTTATAAATGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2608 [new locus tag: SAUSA300_RS14500 ]
  • symbol: HisH
  • description: imidazole glycerol phosphate synthase subunit HisH
  • length: 192
  • theoretical pI: 6.13987
  • theoretical MW: 21279.2
  • GRAVY: -0.0348958

Function[edit | edit source]

  • reaction:
    EC 2.4.2.-?  ExPASy
    5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O?
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 239.6)
    and 7 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 29.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 22.3)
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 18.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 15.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 15.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 14.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 13.5)
  • TheSEED  :
    • Imidazole glycerol-phosphate synthase amidotransferase subunit HisH (EC 4.3.2.10)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)
  • PFAM:
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 64.1)
    and 4 more
    SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 23.7)
    GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 15.6)
    no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 15.4)
    Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 12.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002578
    • TAT(Tat/SPI): 0.000111
    • LIPO(Sec/SPII): 0.000353
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]