From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04855 [old locus tag: SAUSA300_0902 ]
  • pan locus tag?: SAUPAN003171000
  • symbol: SAUSA300_RS04855
  • pan gene symbol?: pepF
  • synonym:
  • product: oligoendopeptidase F

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04855 [old locus tag: SAUSA300_0902 ]
  • symbol: SAUSA300_RS04855
  • product: oligoendopeptidase F
  • replicon: chromosome
  • strand: +
  • coordinates: 989609..991417
  • length: 1809
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGTCAACAATTATCGAGAGAAGAACAGGAACGTAAATATCCTGAATATACATGGGAC
    TTAACAACAATTTTCAAAGATGATGAAGCTTTTGAGGCTGCATTTAAAGAAGTTGAAAAT
    GAGTTAGGCAAAGAAGAACAATTTAAAGGACACATTGGTGATAGTGCTGAGACATTATAC
    AATGCGTTAGAATTAGAAGATACATTAGGTACTAAATTAGAAAAAGTATATGTATACGCG
    CACCTAAAACAAGACCAAGATACAACGAACGACAAGTATACTGGTATGGAGTCAAGAGCA
    CATCAATTAATTATTAAATTTAGCTCGGCATGGAGTTTCTTAGTGCCAGAGATTTTACAA
    ATTGATGAAGATAAAATTCAATCATTTGTAAATTCATATGATAAATTACAAAAATTCGCA
    TTTGATTTGAAGTTGATTAATGAAAAACGTCCTCATATTTTAGATGCTGAAACTGAAAAG
    TTATTAACAGAAGCGCAGGACGCGTTATCAACGCCATCAAATGTATACGGTATGTTTAGC
    AACGCTGATTTAGTATTTGAAGATGCGATAGATAAAGATGGAAATGCACACCCGTTAACA
    CAAGGTACATTTATTAAGTATTTAGAAACAGATGATCGCAAACTAAGAGAAAGTGCTTTT
    AGAAATGTATATAAAGCATATGGTGCTCATAATAATACGCTTGGCGCTACGCTAGCAGGT
    GAAGTGAAGAAAAATGTATTTAATGCTCGTACACACAATTACAAAACTGCAAGAGAAAAA
    GCATTGAGTAATAATCATATTCCAGAAAATGTATATGACAATCTAGTAAAAACTGTACAT
    AAATATTTACCATTGCTACATAGATATACTGAATTGCGCAAAGAATTGCTAGGTTTAGAT
    GACTTGAAAATGTATGATTTATATACACCATTAATTAAAGATATTAAGTTTGAAATGCCT
    TATGAAGAAGCTAAAGAGTGGATGTTAAAAGCATTAGAACCAATGGGTGAAGAATATTTA
    AATGTAGTTAAAGAAGGCTTAAACAATCGTTGGGTCGATGTCTATGAGAATAAAGGTAAA
    CGTTCAGGTGGCTATTCATCAGGTGCACATTTAACTAATCCATTTATTCTACTTAACTGG
    TCTAATACTATTTCAGACTTATACACATTAGTTCATGAATTTGGGCATTCAGCACATAGT
    TACTTCAGTAGAAAATTCCAACCGTCAAATTCTAGTGACTACACTATTTTTGTCGCTGAA
    GTTGCATCAACTTGTAACGAAGCACTTTTAAGTGATTATATGGATAAACATCTTGATGAT
    GAAAAACGCTTATTATTATTAAACCAAGAATTAGAACGTTTCAGAGCTACATTATTCCGA
    CAAACAATGTTCGCAGAATTTGAGCATAAAATTCATGCAATTGAAGAAGCAGGTGAACCA
    TTAACGCCAACTAGAATGAATGAAGAATATGCCAAATTAAATAAATTATACTTCGGTGAT
    TCTGTAGAAACTGATGAAGATATTAGTAAGGAATGGTCACGTATTCCACACTTCTATATG
    AATTATTATGTATATCAATACGCAACTGGTTACAGTGCAGCTCAAAGCTTAAGTCATCAA
    ATTTTAACAGAAGGTAAGCCAGCAGTAGATAGATATATTAATGAATTCTTGAAAAAAGGT
    AGCTCAAATTATCCAATTGAGATATTAAAAAATGCTGGTGTAGATATGACAACACCTGAA
    CCAATTGAACAAGCTTGTGAAGTTTTTGAACAAAAATTGAACGCTTTTGAAAAATTAATG
    AAAGCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1809

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS04855 [old locus tag: SAUSA300_0902 ]
  • symbol: SAUSA300_RS04855
  • description: oligoendopeptidase F
  • length: 602
  • theoretical pI: 4.92265
  • theoretical MW: 69818.9
  • GRAVY: -0.631063

Function[edit | edit source]

  • reaction:
    EC 3.4.24.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides oligoendopeptidase F (TIGR00181; EC 3.4.24.-; HMM-score: 669.1)
    and 2 more
    oligoendopeptidase, pepF/M3 family (TIGR02290; EC 3.4.24.-; HMM-score: 330.2)
    oligoendopeptidase, M3 family (TIGR02289; EC 3.4.24.-; HMM-score: 99.6)
  • TheSEED: see SAUSA300_0902
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M3; Peptidase family M3 (PF01432; HMM-score: 255)
    and 3 more
    no clan defined Peptidase_M3_N; Oligopeptidase F (PF08439; HMM-score: 72.5)
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 13)
    no clan defined DUF2264; Uncharacterized protein conserved in bacteria (DUF2264) (PF10022; HMM-score: 12.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002398
    • TAT(Tat/SPI): 0.00041
    • LIPO(Sec/SPII): 0.000373
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGKEEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQLIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEKLLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRESAFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLVKTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEPMGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVHEFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQELERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDISKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPIEILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]