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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04940 [old locus tag: SAUSA300_0919 ]
  • pan locus tag?: SAUPAN003196000
  • symbol: SAUSA300_RS04940
  • pan gene symbol?: murE
  • synonym:
  • product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04940 [old locus tag: SAUSA300_0919 ]
  • symbol: SAUSA300_RS04940
  • product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1008620..1010101
  • length: 1482
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    TTGGATGCAAGTACGTTGTTTAAGAAAGTAAAAGTAAAGCGTGTATTGGGTTCTTTAGAA
    CAACAAATAGATGATATCACTACTGATTCACGTACAGCGAGAGAAGGTAGCATTTTTGTC
    GCTTCAGTTGGATATACTGTAGACAGTCATAAGTTCTGTCAAAATGTAGCTGATCAAGGG
    TGTAAGTTGGTAGTGGTCAATAAAGAACAATCATTACCAGCTAACGTAACACAAGTGGTT
    GTGCCGGACACATTAAGAGTAGCTAGTATTCTAGCACACACATTATATGATTATCCGAGT
    CATCAGTTAGTGACATTTGGTGTAACGGGTACAAATGGTAAAACTTCTATTGCGACGATG
    ATTCATTTAATTCAAAGAAAGTTACAAAAAAATAGTGCATATTTAGGAACTAATGGTTTC
    CAAATTAATGAAACAAAGACAAAAGGTGCAAATACGACACCAGAAACAGTTTCTTTAACT
    AAGAAAATTAAAGAAGCAGTTGATGCAGGCGCTGAATCTATGACATTAGAAGTATCAAGC
    CATGGCTTAGTATTAGGACGACTGCGAGGCGTTGAATTTGACGTTGCAATATTTTCAAAT
    TTAACACAAGACCATTTAGATTTTCATGGCACAATGGAAGCATACGGACACGCGAAGTCT
    TTATTGTTTAGTCAATTAGGTGAAGATTTGTCGAAAGAAAAGTATGTCGTGTTAAACAAT
    GACGATTCATTTTCTGAGTATTTAAGAACAGTGACGCCTTATGAAGTATTTAGTTATGGA
    ATTGATGAGGAAGCCCAATTTATGGCTAAAAATATTCAAGAATCTTTACAAGGTGTCAGC
    TTTGATTTTGTAACGCCTTTTGGAACTTACCCAGTAAAATCGCCTTATGTTGGTAAGTTT
    AATATTTCTAATATTATGGCGGCAATGATTGCGGTGTGGAGTAAAGGTACATCTTTAGAA
    ACGATTATTAAAGCTGTTGAAAATTTAGAACCTGTTGAAGGGCGATTAGAAGTTTTAGAT
    CCTTCGTTACCTATTGATTTAATTATCGATTATGCACATACAGCTGATGGTATGAACAAA
    TTAATCGATGCAGTACAGCCTTTTGTAAAGCAAAAGTTGATATTTTTAGTTGGTATGGCA
    GGCGAACGTGATTTAACTAAAACGCCTGAAATGGGGCGAGTTGCCTGTCGTGCAGATTAT
    GTCATTTTCACACCGGATAATCCGGCAAATGATGACCCGAAAATGTTAACGGCAGAATTA
    GCCAAAGGTGCAACACATCAAAACTATATTGAATTTGATGATCGTGCAGAAGGGATAAAA
    CATGCAATTGACATAGCTGAGCCTGGGGATACTGTCGTTTTAGCATCAAAAGGAAGAGAA
    CCATATCAAATCATGCCAGGGCATATTAAGGTGCCACATCGAGATGATTTAATTGGCCTT
    GAAGCAGCTTACAAAAAGTTCGGTGGTGGCCCTGTTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

Protein[edit | edit source]

Protein Data Bank: 4C12
Protein Data Bank: 4C13

General[edit | edit source]

  • locus tag: SAUSA300_RS04940 [old locus tag: SAUSA300_0919 ]
  • symbol: SAUSA300_RS04940
  • description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase
  • length: 493
  • theoretical pI: 5.33961
  • theoretical MW: 54104.2
  • GRAVY: -0.1714

Function[edit | edit source]

  • reaction:
    EC 6.3.2.7?  ExPASy
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-lysine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 432)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 135.4)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 95.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 90.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 80.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 41.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 26.4)
  • TheSEED: see SAUSA300_0919
  • PFAM:
    no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 179.9)
    and 2 more
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 65.6)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 29.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014761
    • TAT(Tat/SPI): 0.000832
    • LIPO(Sec/SPII): 0.001006
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

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    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]