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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07965 [old locus tag: SAUSA300_1458 ]
  • pan locus tag?: SAUPAN004046000
  • symbol: SAUSA300_RS07965
  • pan gene symbol?:
  • synonym:
  • product: glyoxalase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07965 [old locus tag: SAUSA300_1458 ]
  • symbol: SAUSA300_RS07965
  • product: glyoxalase
  • replicon: chromosome
  • strand: -
  • coordinates: 1612529..1612900
  • length: 372
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGAAAAGTTAAATCAAGTTATGTTATATGTTGATGATGTCGAAAAAGCGAAAGCATTT
    TGGACAGAAACTTTAGAATTTGTTGTTGTAAGTGAAACACCGTTAGCTGAGGACTATGTT
    GCAGTTGAAGTATCACCTACCAAAGATGCTGAAACGTCATTGACGATTATGGCAAAAGAA
    TTTATTGAAAAGCATAGCCCGGAAGTGAACCTAGGAACACCATCTTTAATGTTTAAAGAG
    AAAAACTTTGATGCATTATACTCAAAATTAAATGATTTAGGTTTAACTGGGCATGATATA
    GTAGAAATGAATGGTCAACGTGTATTTAATTTTCAAGATGGCCAAGGTAACTATTTTGCA
    GTTAGTGACTAA
    60
    120
    180
    240
    300
    360
    372

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS07965 [old locus tag: SAUSA300_1458 ]
  • symbol: SAUSA300_RS07965
  • description: glyoxalase
  • length: 123
  • theoretical pI: 4.1167
  • theoretical MW: 13890.6
  • GRAVY: -0.255285

Function[edit | edit source]

  • reaction:
    EC 4.4.1.5?  ExPASy
    Lactoylglutathione lyase (R)-S-lactoylglutathione = glutathione + methylglyoxal
  • TIGRFAM:
    methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 16.4)
  • TheSEED: see SAUSA300_1458
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 32.6)
    and 3 more
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 26)
    Glyoxalase_4; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF13669; HMM-score: 16.2)
    no clan defined DUF2481; Protein of unknown function (DUF2481) (PF10654; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005495
    • TAT(Tat/SPI): 0.000318
    • LIPO(Sec/SPII): 0.000432
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEVSPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVEMNGQRVFNFQDGQGNYFAVSD

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]