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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
  • pan locus tag?: SAUPAN005845000
  • symbol: SAUSA300_RS12665
  • pan gene symbol?: fni
  • synonym:
  • product: type 2 isopentenyl-diphosphate Delta-isomerase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
  • symbol: SAUSA300_RS12665
  • product: type 2 isopentenyl-diphosphate Delta-isomerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2464602..2465651
  • length: 1050
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGTGATTTTCAAAGAGAACAGAGAAAAAATGAACATGTTGAAATAGCAATGGCCCAA
    TCTGACGCAATGCATTCAGATTTTGATAAGATGCGTTTTGTGCATCATTCTATCCCATCA
    ATTAATGTAAATGATATCGATTTGACATCACAGACGCCTGACTTAACGATGGCATATCCG
    GTTTATATTAATGCAATGACGGGTGGTAGCGAGTGGACGAAAAACATCAATGAAAAGCTA
    GCTGTAGTTGCAAGAGAAACTGGCTTAGCGATGGCAGTTGGATCAACACATGCGGCATTG
    AGAAATCCACGCATGGCTGAGACGTTTACGATTGCGCGAAAAATGAATCCTGAAGGCATG
    ATTTTTAGCAATGTTGGTGCGGACGTACCAGTAGAAAAGGCTTTGGAAGCAGTTGAATTA
    CTTGAGGCACAAGCGTTACAAATCCATGTTAATTCTCCTCAAGAATTAGTTATGCCTGAA
    GGGAATCGTGAATTTGTGACTTGGTTAGATAATATAGCGTCGATTGTATCACGAGTGTCT
    GTTCCAGTCATTATAAAAGAAGTTGGATTTGGTATGAGCAAAGAATTAATGCATGACTTA
    CAACAAATAGGCGTCAAGTATGTCGATGTTAGTGGCAAAGGTGGTACTAACTTTGTAGAT
    ATTGAAAATGAACGTCGTGCAAATAAAGATATGGATTACTTATCATCATGGGGACAGTCT
    ACAGTTGAGTCATTACTTGAAACAACGGCTTATCAAAGCGAAATTTCAGTTTTCGCGAGT
    GGTGGTTTACGTACACCACTCGATGCAATTAAAAGTTTAGCACTTGGTGCAAAGGCAACT
    GGTATGTCACGTCCGTTTTTAAATCAAGTTGAAAATAATGGCATTGCACATACAGTAGCT
    TATGTAGAATCATTTATTGAACACATGAAATCAATAATGACGATGTTAGATGCTAAAAAT
    ATTGACGACTTAACACAAAAACAAATCGTATTTAGTCCGGAAATATTGTCATGGATAGAA
    CAACGTAACTTGAATATACATCGAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1050

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
  • symbol: SAUSA300_RS12665
  • description: type 2 isopentenyl-diphosphate Delta-isomerase
  • length: 349
  • theoretical pI: 5.0077
  • theoretical MW: 38769.9
  • GRAVY: -0.201719

Function[edit | edit source]

  • reaction:
    EC 5.3.3.2?  ExPASy
    Isopentenyl-diphosphate Delta-isomerase Isopentenyl diphosphate = dimethylallyl diphosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 426.4)
    and 6 more
    heme/flavin dehydrogenase, mycofactocin system (TIGR03966; EC 1.-.-.-; HMM-score: 28.6)
    Metabolism Energy metabolism Other L-lactate oxidase (TIGR02708; EC 1.1.3.-; HMM-score: 16.2)
    Metabolism Energy metabolism Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 14.8)
    putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 13.9)
  • TheSEED: see SAUSA300_2292
  • PFAM:
    TIM_barrel (CL0036) FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 85.5)
    and 3 more
    Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 25.8)
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 17.7)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN, Mg2+, NADPH
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008927
    • TAT(Tat/SPI): 0.001746
    • LIPO(Sec/SPII): 0.001146
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVNDIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRNPRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPEGNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNFVDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALGAKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPEILSWIEQRNLNIHRG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]