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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13800 [old locus tag: SAUSA300_2485 ]
  • pan locus tag?: SAUPAN006209000
  • symbol: SAUSA300_RS13800
  • pan gene symbol?:
  • synonym:
  • product: methylated-DNA--[protein]-cysteine S-methyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13800 [old locus tag: SAUSA300_2485 ]
  • symbol: SAUSA300_RS13800
  • product: methylated-DNA--[protein]-cysteine S-methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2684265..2684786
  • length: 522
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAGTATAAGAGTTACTATGATTCGCCTGTAGGACGATTGGAACTTTTGAGTGATGGG
    GTAAGTCTGACCGCTGTTTTGTTTGAAAATCAACAAGGTGATGGTACTAGGGAAGAAAAT
    ACGTCTTTAGCGATATTTAGAGAGGCGACACAGTGGTTAGACGCGTATTTTAAAGGTGAC
    AATCCTGAAATCACAATACCTTTAAAGCCAACAGGTAGTCATTTTCAACAGTGTGTTTGG
    AATGAATTAAGACAGGTTCCTTATGGGACTCTAACAACGTATGGTGCTATTGCCAAAAAA
    GTAGGCAAGCTGCTTAACAAGCCAAAAATGTCAGCCCAAGCGGTTGGAGGTGCAGTCGGA
    AGTAATCCATTATCTATTATCGTGCCATGTCATCGTGTTGTCGGTAAAACGGGTAGCTTA
    ACAGGGTTCGGTGGAACGATTAATAATAAAATTAAGCTGTTAGAGTTGGAGAATATCGAT
    ATGTCAAAGCTTTATGTACCAAAACATAGCACGAAGCCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    522

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS13800 [old locus tag: SAUSA300_2485 ]
  • symbol: SAUSA300_RS13800
  • description: methylated-DNA--[protein]-cysteine S-methyltransferase
  • length: 173
  • theoretical pI: 9.01352
  • theoretical MW: 18945.6
  • GRAVY: -0.29422

Function[edit | edit source]

  • reaction:
    EC 2.1.1.63?  ExPASy
    Methylated-DNA--[protein]-cysteine S-methyltransferase DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair methylated-DNA--[protein]-cysteine S-methyltransferase (TIGR00589; EC 2.1.1.63; HMM-score: 92.5)
  • TheSEED: see SAUSA300_2485
  • PFAM:
    no clan defined DNA_binding_1; 6-O-methylguanine DNA methyltransferase, DNA binding domain (PF01035; HMM-score: 103.1)
    and 1 more
    Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain (PF02870; HMM-score: 30.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006297
    • TAT(Tat/SPI): 0.000595
    • LIPO(Sec/SPII): 0.001318
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLAIFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVGKLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]