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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS14445 [old locus tag: SAUSA300_2598 ]
  • pan locus tag?: SAUPAN006409000
  • symbol: SAUSA300_RS14445
  • pan gene symbol?: cap1A
  • synonym:
  • product: capsular polysaccharide biosynthesis protein CapA

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS14445 [old locus tag: SAUSA300_2598 ]
  • symbol: SAUSA300_RS14445
  • product: capsular polysaccharide biosynthesis protein CapA
  • replicon: chromosome
  • strand: -
  • coordinates: 2824377..2825039
  • length: 663
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGAAAAGTTTGATTTAGTAAAACTATTAAATATTCTAAAGAAGAATATTAAATTA
    TTGCTTATTTTACCGGCAATATGTCTTGTAGTAAGTGCGGCATTAACCTTTTTTGTTATG
    CCTGACAAATATACTGCTTCTACTCAAATATTAGTGAACATGAAAAAGTCCTCAAGTGAT
    TTAGCTTTCCAAAATGTTCAAAGTAGTTTGCAGTCTGTTAATACATATACGGAAATTATC
    AAAAGTCCTAGAATTCTAGATAAAGTATCTAGAGAATTTGATGGTCAGTATTCAACAGCT
    GAGTTGAATTCATTTTTAAAAGTAACCAATCAAACAAACTCTCAAATTATTACTGTATCA
    GTTACCACTGGGAATAAATCTGAATCTGACAAAATTGTTAATAAAATATCTAAAGTTTTT
    GCTCATGACATGCCTAAAATTATGAGTGTTGATAATGTTACGATACTTTCCTCAGCACAT
    GATAATGCTGTAAAAGTATCTCCAATAGTATCTGTCAACTTAGTTATCAGCATTATTGTT
    GGTATTGTTTTAGCAATATTGATTATTTTCTTAAAAGAATTATTAGATAAGCGTATTAAG
    ACAGAAGAAGATGTTGAATCGCAACTAGGATTACCTATTTTAGGTTCAATACAAAAATTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS14445 [old locus tag: SAUSA300_2598 ]
  • symbol: SAUSA300_RS14445
  • description: capsular polysaccharide biosynthesis protein CapA
  • length: 220
  • theoretical pI: 9.8166
  • theoretical MW: 24339.5
  • GRAVY: 0.299091

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polysaccharide export protein, MPA1 family (TIGR01006; HMM-score: 240.8)
    and 4 more
    chain length determinant protein EpsF (TIGR03017; HMM-score: 55.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 41.8)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (TIGR03007; HMM-score: 29.4)
    NDMA-dependent methanol dehydrogenase (TIGR04266; EC 1.1.99.37; HMM-score: 15.4)
  • TheSEED: see SAUSA300_2598
  • PFAM:
    no clan defined Wzz; Chain length determinant protein (PF02706; HMM-score: 82.6)
    and 7 more
    GNVR; G-rich domain on putative tyrosine kinase (PF13807; HMM-score: 20.1)
    Amnionless; Amnionless (PF14828; HMM-score: 13.9)
    DUF2975; Protein of unknown function (DUF2975) (PF11188; HMM-score: 13.7)
    CD34_antigen; CD34/Podocalyxin family (PF06365; HMM-score: 12.6)
    VSA (CL0656) RIFIN; Rifin (PF02009; HMM-score: 11.1)
    FUSC (CL0307) FUSC; Fusaric acid resistance protein family (PF04632; HMM-score: 8.8)
    no clan defined DUF5362; Family of unknown function (DUF5362) (PF17319; HMM-score: 8.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.154438
    • TAT(Tat/SPI): 0.003094
    • LIPO(Sec/SPII): 0.028865
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKYTASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAELNSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAHDNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQKF

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]