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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS14795 [old locus tag: SAUSA300_pUSA030012 ]
  • pan locus tag?:
  • symbol: SAUSA300_RS14795
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS14795 [old locus tag: SAUSA300_pUSA030012 ]
  • symbol: SAUSA300_RS14795
  • product: hypothetical protein
  • replicon: pUSA03
  • strand: +
  • coordinates: 11407..11814
  • length: 408
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGTCTGTTGAGCAAGAAGTTAAAGATATAATGCTCGATATGTTGGGCGAAGAAAAGAAA
    GTTAAAGAGAATATAATACCTCGTAATGTAGACGCTAGTTATAAAATATTCTTAAATTTA
    AACGGTAAAGAATTAGCAATTGTATTTGTTCCTTTTTTACTATTTCTAACAATAGCATTT
    GGTGTAACGTTTTTAATGGGCTTACTTAATTTAATAACTGGCTTTATGGTTTTTATAGTA
    GGTTTGATTTTTGGTTTAACAATATATGGCTTACTAACAATTAGACCAATTAGTACAAAA
    GAAAATATAAGAATGATTGATACCATAAAACAATCACAACGATTTAGCAGAAGGCAGAAA
    GTTTACTTCTATAAAAGTAAAGAAGGATTGGGTGATGATGATGTTTAA
    60
    120
    180
    240
    300
    360
    408

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS14795 [old locus tag: SAUSA300_pUSA030012 ]
  • symbol: SAUSA300_RS14795
  • description: hypothetical protein
  • length: 135
  • theoretical pI: 9.41803
  • theoretical MW: 15476.3
  • GRAVY: 0.331852

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides intracellular adhesion protein D (TIGR03932; HMM-score: 11.2)
  • TheSEED: see SAUSA300_pUSA030012
  • PFAM:
    no clan defined PrgI; PrgI family protein (PF12666; HMM-score: 19.1)
    and 9 more
    Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 15)
    YwcE; Spore morphogenesis and germination protein YwcE (PF17368; HMM-score: 14.3)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 13.7)
    DUF2149; Uncharacterized conserved protein (DUF2149) (PF09919; HMM-score: 13)
    Myc_target_1; Myc target protein 1 (PF15179; HMM-score: 12.4)
    YuiB; Putative membrane protein (PF14068; HMM-score: 9.6)
    APC (CL0062) AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 9.4)
    no clan defined Promethin; Promethin (PF16015; HMM-score: 7.3)
    DUF5469; Family of unknown function (DUF5469) (PF17561; HMM-score: 7.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004948
    • TAT(Tat/SPI): 0.000137
    • LIPO(Sec/SPII): 0.002723
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSVEQEVKDIMLDMLGEEKKVKENIIPRNVDASYKIFLNLNGKELAIVFVPFLLFLTIAFGVTFLMGLLNLITGFMVFIVGLIFGLTIYGLLTIRPISTKENIRMIDTIKQSQRFSRRQKVYFYKSKEGLGDDDV

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]