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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030004 [new locus tag: SAUSA300_RS14755 ]
  • pan locus tag?:
  • symbol: SAUSA300_pUSA030004
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_pUSA030004 [new locus tag: SAUSA300_RS14755 ]
  • symbol: SAUSA300_pUSA030004
  • product: hypothetical protein
  • replicon: pUSA03
  • strand: +
  • coordinates: 5306..5704
  • length: 399
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGTATAATTTTGATTATTCTAAATTACCAATTAAAAATATTCAAAAAATATTTCCTATT
    GCAGGGGGATATGTGAATCTATCTTTTTCAGTTGATGCTTCCAACAAAAAATATTTTTTA
    AAGCTACAACCTAATACTAAATCAAATTTTTTTGATTATGAGTTATCCAGTTTAAAAGAA
    TTAACTGATAAAAATATTCCTGTTCCTCAAATTATTAATAAAGGAGAATTAGACAATAAT
    TCTTTTTTACTGTTAGAGTTCATTGAAAATGGCCATGCTTATCCTGAAAGCTATAGAAAA
    CTAGGTAAAATAGTTGCAAACATGCATAAAAATATAAATTCACTAAATCTATTTGGCTTT
    AGTCATAATTTCGGTTCTGTTGCAAAGTTGAATTTATAG
    60
    120
    180
    240
    300
    360
    399

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_pUSA030004 [new locus tag: SAUSA300_RS14755 ]
  • symbol: SAUSA300_pUSA030004
  • description: hypothetical protein
  • length: 132
  • theoretical pI: 9.70889
  • theoretical MW: 15100.2
  • GRAVY: -0.297727

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Fructosamine-3-kinase (EC 2.7.1.-)
  • PFAM:
    PKinase (CL0016) Fructosamin_kin; Fructosamine kinase (PF03881; HMM-score: 71.4)
    and 2 more
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 28.6)
    Kdo; Lipopolysaccharide kinase (Kdo/WaaP) family (PF06293; HMM-score: 11.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.072264
    • TAT(Tat/SPI): 0.001778
    • LIPO(Sec/SPII): 0.002567
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQPNTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLGKIVANMHKNINSLNLFGFSHNFGSVAKLNL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]