From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13530 [old locus tag: SA2361 ]
  • pan locus tag?: SAUPAN006248000
  • symbol: SA_RS13530
  • pan gene symbol?:
  • synonym:
  • product: glyoxalase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13530 [old locus tag: SA2361 ]
  • symbol: SA_RS13530
  • product: glyoxalase
  • replicon: chromosome
  • strand: -
  • coordinates: 2652919..2653296
  • length: 378
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2652919..2653296) NCBI
  • BioCyc: G1G21-2748 BioCyc
  • MicrobesOnline: see SA2361

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGATTCAATCAATGTGGTTTAATTTGCATGTGCAAGATTTAGAAAAGAGCGCACAGTTT
    TATAAAGCGTTAGGATTTAAAATAAATAGAAACCCACAAATGTTAGATAAAATGGTCGGT
    ATTCAAATAGGTCAAACAACCGTAATTTTAATAGAAAACAAGCATTTTCAAAATGTAAGT
    CAGCAAAGCCTTAATACTGAACCAAATGAAGTGATGATTTCTCTAGGTGTGAACACAAAT
    GAAGAAGTTGACCAGTTGGTGAATAAAGTGAAAGAAGCGGGTGGCGCAGTCGTTCAAGAA
    CCAACAGTAAGCCAAGGGTTTTATGGTGCAATGTTCAAAGATCTTGATGGACACCATTTT
    AATTTTTTAGTCTGCTAA
    60
    120
    180
    240
    300
    360
    378

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS13530 [old locus tag: SA2361 ]
  • symbol: SA_RS13530
  • description: glyoxalase
  • length: 125
  • theoretical pI: 5.54363
  • theoretical MW: 14130.1
  • GRAVY: -0.2024

Function[edit | edit source]

  • TIGRFAM:
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 23.6)
    Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 22.9)
    Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 22.9)
  • TheSEED: see SA2361
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 32.2)
    and 4 more
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 14.2)
    Glyoxalase_4; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF13669; HMM-score: 14.1)
    3-dmu-9_3-mt; 3-demethylubiquinone-9 3-methyltransferase (PF06983; HMM-score: 13.5)
    Peptidase_MH (CL0035) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002114
    • TAT(Tat/SPI): 0.000315
    • LIPO(Sec/SPII): 0.000431
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIGQTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGAVVQEPTVSQGFYGAMFKDLDGHHFNFLVC

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]