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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS09405 [old locus tag: SA1664 ]
  • pan locus tag?: SAUPAN004777000
  • symbol: SA_RS09405
  • pan gene symbol?: —
  • synonym:
  • product: DUF445 domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS09405 [old locus tag: SA1664 ]
  • symbol: SA_RS09405
  • product: DUF445 domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1900202..1901326
  • length: 1125
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1900202..1901326) NCBI
  • BioCyc: SA_RS09405 BioCyc
  • MicrobesOnline: see SA1664

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATGCACTATTTATTATCATATTTATGATTGTAGTAGGGGCGATAATTGGGGGCATT
    ACTAATGTAATTGCAATTAGAATGCTGTTTCACCCCTTTAAACCATACTATATATTTAAA
    TTTAGAGTCCCATTTACACCAGGTTTAATACCGAAAAGACGCGAAGAAATTGCAACTAAA
    ATTGGCCAAGTGATTGAAGAGCATTTGCTTACAGAAACTTTAATTAATGAAAAATTAAAA
    AGCGAGCAATCACAGCAAGCAATAGAATCTATGATTCAACAGCAGTTACAAAAGTTGACG
    AAAGATCAATTGTCAATAAAACAAATTACTTCTCAAATCGATATTGATTTAGAACAAGTA
    TTACAAACTAATGGAAATCAATATATTGAATCACAATTGAATAATTATTATACAAAGCAT
    CAAAACCAAACAATAGCATCTTTATTGCCAAATCAACTTGTAACATTTTTAGATCAGCAT
    GTAGATAACGCAACAGACTTATTATGTGATCGTGCAAGGAATTATTTATCATCTGCAAAA
    GGCACACAAGATATTAATGATATGTTGGATACTTTTTTCCATGAGAAAGGTAAGTTAATT
    GGTATGTTGCAAATGTTTATGACAAAAGAGAGCATTGCAGATCGCATTCAACAAGAACTT
    ATACGTTTAACATCTCATCCTAAAGCAAGAACAATTGTGACATCGTTAATTACCAATGAA
    TATCAAACTTTTAAAGATAAACCATTGAATGAACTGTTAGATGCATCGCAGTTTAATGAA
    ATCGCTGAAAATCTATCAGTGTATGTAACAACATATGCAAGCAATCAAGCGAATAAGCCA
    GTGGTTACGTTAATGCCTCAATTCGTTGATTATTTAGAAGGTCAACTCTCTAGCAAATTA
    GCAAATTTAATCATTGAAAAATTATCGATTCACTTATCTACAATTATGAAAAAAGTGGAT
    TTACGAGGATTAATTGAAGAACAAATTAATACGTTTGACTTAGATTATATTGAAAAATTA
    ATTATAGAAATTGCTAACAAAGAACTAAAGTTGATTATGTCATTAGGTTTCATTTTAGGT
    GGTATTATTGGATTTTTCCAAGGTTTGGTTGCAATCTTTGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS09405 [old locus tag: SA1664 ]
  • symbol: SA_RS09405
  • description: DUF445 domain-containing protein
  • length: 374
  • theoretical pI: 5.93545
  • theoretical MW: 42705.4
  • GRAVY: 0.0136364

⊟Function[edit | edit source]

  • TIGRFAM:
    conserved hypothetical protein (TIGR04540; HMM-score: 4.4)
  • TheSEED: see SA1664
  • ⊞PFAM:
    no clan defined DUF445; Protein of unknown function (DUF445) (PF04286; HMM-score: 251.4)
    and 5 more
    CC_BshC; Bacillithiol biosynthesis BshC, C-terminal coiled-coil domain (PF24850; HMM-score: 17.8)
    Dim_A_B_barrel (CL0032) Cyclase_polyket; Polyketide synthesis cyclase (PF04673; HMM-score: 12.3)
    no clan defined STAT_int; STAT protein, protein interaction domain (PF02865; HMM-score: 11.8)
    WWE (CL0783) WWE; WWE domain (PF02825; HMM-score: 9.2)
    PDDEXK (CL0236) Dna2; DNA replication factor Dna2 (PF08696; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0085
    • Cytoplasmic Membrane Score: 0.9907
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0006
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.066344
    • TAT(Tat/SPI): 0.001417
    • LIPO(Sec/SPII): 0.042124
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVPFTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKDQLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQHVDNATDLLCDRARNYLSSAKGTQDINDMLDTFFHEKGKLIGMLQMFMTKESIADRIQQELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASNQANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDLDYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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