PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 22-FEB-2026

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: EGJ38_RS08590 [old locus tag: EGJ38_001720 ]
• pan locus tag^?: SAUPAN004396000
• symbol: EGJ38_RS08590
• pan gene symbol^?: fhs
• synonym:
• product: formate--tetrahydrofolate ligase

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: EGJ38_RS08590 [old locus tag: EGJ38_001720 ]
• symbol: EGJ38_RS08590
• product: formate--tetrahydrofolate ligase
• replicon: chromosome
• strand: -
• coordinates: 1763033..1764700
• length: 1668
• essential: unknown other strains

⊟Accession numbers

• Location: NZ_CM129921 (1763033..1764700) NCBI
• BioCyc:
• MicrobesOnline:

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

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  1621
  
  TTGACTCATTTATCAGATTTAGATATTGCGAATCAATCAACACTACAACCAATTAAGGAT
  ATTGCTGCATCAGTAGGTATTTCAGAGGATGCATTAGAACCTTATGGTCATTACAAAGCT
  AAAATCGACATTAATAAAATTACGCCAAGAGAAAACAAAGGGAAAGTTGTTTTAGTAACT
  GCGATGAGCCCAACACCAGCTGGTGAAGGTAAATCAACGGTTACAGTTGGTTTAGCTGAT
  GCATTCCATGAGTTAAATAAAAACGTTATGGTTGCATTAAGAGAGCCTGCTTTAGGACCA
  ACATTTGGTATCAAAGGTGGTGCGACTGGTGGTGGTTATGCGCAAGTCTTACCTATGGAA
  GATATCAACTTACATTTCAACGGAGATTTCCATGCGATTACAACTGCAAATAATGCATTG
  TCTGCGTTTATCGATAATCATATTCACCAAGGTAACGAATTAGGAATCGATCAAAGACGT
  ATTGAGTGGAAACGTGTATTAGATATGAATGATCGTGCACTTAGACATGTAAACGTTGGG
  TTAGGTGGACCTACAAATGGTGTACCACGTGAAGATGGCTTTAATATTACAGTAGCGTCT
  GAAATTATGGCGATTTTATGTTTAAGTAGAAGTATTAAAGACTTAAAAGATAAAATTAGT
  CGTATTACTATTGGTTACACTAGAGATCGCAAGCCAGTTACAGTTGCAGATTTAAAAGTG
  GAAGGTGCACTTGCAATGATTTTAAAAGATGCAATAAAACCAAACTTAGTACAATCAATT
  GAAGGGACACCTGCATTAGTTCATGGTGGACCATTTGCGAATATCGCACACGGTTGTAAC
  TCAATTTTAGCAACTGAAACAGCACGTGATTTAGCTGATATCGTTGTAACGGAAGCTGGA
  TTTGGTTCAGACTTAGGCGCTGAAAAATTCATGGATATTAAAGCGCGTGAAGCAGGATTT
  GATCCGGCAGCTGTCGTTGTTGTTGCGACAATTCGTGCGTTAAAAATGCATGGTGGTGTA
  GCGAAAGATAATTTAAAAGAAGAAAATGTAGAAGCAGTAAAAGCAGGAATTGTTAATTTA
  GAGCGTCATGTTAATAATATTAAAAAATTCGGTGTAGAACCGGTTGTTGCAATTAATGCA
  TTTATACATGATACCGATGCAGAAGTAGAATATGTAAAATCTTGGGCTAAAGAAAATAAC
  GTACGAATTGCCTTAACTGAAGTTTGGGAAAAAGGTGGTAAAGGTGGCGTTGACTTAGCA
  AATGAAGTATTAGAAGTCATTGATCAACCTAATTCATTTAAACCTTTATATGAATTAGAA
  TTACCATTAGAGCAAAAGATTGAAAAGATTGTGACTGAAATCTATGGCGGTTCAAAAGTA
  ACGTTTAGCAGTAAAGCGCAAAAACAATTAAAACAATTTAAAGAAAATGGTTGGGATAAT
  TACCCAGTATGTATGGCGAAAACACAATATTCATTCTCAGATGATCAAACGTTGTTAGGT
  GCACCATCAGGATTTGAAATTACAATTCGTGAATTAGAAGCGAAAACAGGTGCAGGATTT
  ATCGTAGCGTTGACAGGTGCAATCATGACTATGCCTGGTTTACCTAAAAAACCAGCAGCA
  TTAAACATGGATGTTACTGATGATGGTCATGCAATTGGGTTATTCTAA
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  1668
  

⊟Protein

⊟General

• locus tag: EGJ38_RS08590 [old locus tag: EGJ38_001720 ]
• symbol: EGJ38_RS08590
• description: formate--tetrahydrofolate ligase
• length: 555
• theoretical pI: 5.77686
• theoretical MW: 59856.1
• GRAVY: -0.115135

⊟Function

• reaction:
  EC 6.3.4.3?  ExPASy
  Formate--tetrahydrofolate ligase ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 890.7)
  and 2 more
  POTRA (CL0191) POTRA_TamA_1; POTRA domain TamA domain 1 (PF17243; HMM-score: 14.7)
  P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9127
  • Cytoplasmic Membrane Score: 0.0022
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0851
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005497
  • TAT(Tat/SPI): 0.001617
  • LIPO(Sec/SPII): 0.001074
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000149403 NCBI
• UniProt:

⊟Additional information (user-provided)

⊟Protein sequence

• MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDINKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTFGIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRRIEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDKISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIAHGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRALKMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYVKSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see EGJ38_001720

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications