From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000138
  • pan locus tag?: SAUPAN001040000
  • symbol: JSNZ_000138
  • pan gene symbol?: hsdR
  • synonym:
  • product: type I restriction endonuclease subunit R

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000138
  • symbol: JSNZ_000138
  • product: type I restriction endonuclease subunit R
  • replicon: chromosome
  • strand: +
  • coordinates: 164410..166683
  • length: 2274
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCATACCAAAGTGAATACGCATTAGAAAATGAAATGATGAATCAACTTGAACAATTG
    GGTTACGAAAGAGTAACAATACGTGATAATAAGCAATTGCTTGATAATTTTAGAACGATT
    TTAAATGAGCGTCATGCGGACAAATTAGAAGGCAATCCCTTAACAGATAAAGAATTTCAA
    CGTCTGTTAACGATGATTGATGGGAAAAGTATTTTCGAGAGTGCCCGTATTTTACGTGAT
    AAATTACCACTTAGACGTGATGATGAGTCTGAGATTTATTTGTCGTTTTTAGATACGAAA
    AGTTGGTGTAAAAATAAGTTTCAAGTGACGAATCAAGTATCTGTCGAGGATACATATAAA
    GCACGTTATGATGTAACGATATTAATCAACGGACTACCCCTTGTCCAAGTTGAATTGAAA
    CGTCGAGGTATTGATATTAATGAGGCGTTTAACCAAGTAAAACGTTACCGCAAACAAAAT
    TACACAGGCTTATTCCGCTACATACAAATGTTTATCATTAGTAATGGTGTTGAAACGCGA
    TACTTTTCTAATAATGATAGCGAACTATTGAAGAGTCACATGTTTTATTGGAGTGATAAA
    CAGAATAACCGAATCAATACATTACAATCATTTGCTGAGTCATTTATGAGACCCTGTCAA
    TTAGCTAAGATGATATCGCGCTATATGATTATTAATGAAACAGATAGAATACTGATGGCA
    ATGCGTCCGTATCAAGTGTATGCGGTAGAAGCACTTATTCAACAAGCGACTGAGACAGGG
    AATAATGGATATGTATGGCATACAACTGGAAGTGGTAAGACATTGACTTCTTTTAAAGCG
    AGTCAGATTTTATCACAGCAAGATGACATTAAGAAAGTTATCTTTTTGGTTGACCGTAAA
    GACTTGGATAGTCAAACAGAAGAGGAATTTAATAAATTTGCTAAGGGTGCTGTAGACAAA
    ACTTTTAATACCTCGCAACTGGTACGCCAACTAAATGATAAAAGTTTGCCACTTATTGTA
    ACGACGATTCAAAAAATGGCTAAAGCGATTCAAGGGAATGCCCATTTATTAGAACAGTAT
    AAAACGAATAAAGTTGTATTTATTATTGATGAGTGTCATCGCAGTCAATTTGGTGACATG
    CATCGTCTAGTTAAACAACATTTCAAAAATGCCCAATACTTTGGGTTCACTGGCACGCCA
    CGTTTCCCAGAAAATAGTAGTCAAGATGGTAGAACAACTGCAGATATTTTCGGTAGATGC
    TTACATACGTATTTAATTAGAGATGCCATTCATGATGGTAATGTACTTGGTTTCTCAGTT
    GACTATATTAATACTTTTAAAAATAAAGCTTTAAAAGCAGAAGATAACAGCATGGTTGAA
    GCAATTGATACGGAAGAAGTATGGTTAGCGGATAAACGTGTGGAATTAGTAACACGACAT
    ATCATCAATAATCATGATAAATATACACGTAATCGTCAATATTCAAGTATATTTACAGTC
    CAAAGTATTCACGCGCTTATTAAATATTATGAGACATTTAAGCGACTTAACAAAAAGTTG
    GAACAACCATTAACGGTAGCTGGTATATTTACGTTTAAACCTAATGAAGATGATCGTGAT
    GGTGAAGTGCCATATCATTCACGTGAAAAATTAGAGATAATGATTAGCGATTATAATAAA
    AAGTTCGAGACGAATTTTTCAACAGACACAACTAATGAGTATTTTAATCATATTTCAAAA
    AACGTTAAAAAGGGCGTTAAAGATAGTAAAATTGATATCTTAATCGTTGTTAATATGTTC
    TTAACTGGTTTTGATAGTAAAGTACTGAACACTTTATATGTTGACAAGAATTTAATGTAT
    CATGATTTAATTCAAGCGTATTCACGTACAAATAGGGTTGAAAAAGAATCAAAGCCATTT
    GGTAAAATTGTAAACTATCGTGACTTGAAAAAAGAGACAGACGATGCACTGAGAGTATTC
    TCACAAACAAATGATACGGATACAATTTTAATGCGTAGTTATGAAGAGTATAAAAAAGAA
    TTTATGGACGCTTATCGTGAGCTTAAAATGATTGTGCCGACACCACACATGGTTGATGAC
    ATTCAAGATGAACAAGCCCTAAAAACAATTTCAAATGACTACCAGTATAGTGGTGTTGTA
    AACCCACATCACCTTAAAAAAATGATTGGTGATTTGCCATTGAAAGAAAAACGTAAAGCA
    AGAAAAGCCATTGAATCTTTCGTGGCAGAAACAACTGAAAAATACGGTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2274

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000138
  • symbol: JSNZ_000138
  • description: type I restriction endonuclease subunit R
  • length: 757
  • theoretical pI: 8.72708
  • theoretical MW: 88681.2
  • GRAVY: -0.62074

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.21.3?  ExPASy
    Type I site-specific deoxyribonuclease Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I site-specific deoxyribonuclease, HsdR family (TIGR00348; EC 3.1.21.3; HMM-score: 461.1)
    and 1 more
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 36.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) SWI2_SNF2; SWI2/SNF2 ATPase (PF18766; HMM-score: 203.4)
    T1R_D3-like; Type I restriction enzyme subunit R domain 3 (PF22679; HMM-score: 192)
    and 6 more
    PDDEXK (CL0236) HSDR_N; Type I restriction enzyme R protein N terminus (HSDR_N) (PF04313; HMM-score: 147.7)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 137)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 46.9)
    no clan defined EcoR124_C; Type I restriction and modification enzyme - subunit R C terminal (PF12008; HMM-score: 28.6)
    P-loop_NTPase (CL0023) SNF2-rel_dom; SNF2-related domain (PF00176; HMM-score: 17.7)
    NTN (CL0052) GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 15.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7097
    • Cytoplasmic Membrane Score: 0.1205
    • Cell wall & surface Score: 0.0027
    • Extracellular Score: 0.1671
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001624
    • TAT(Tat/SPI): 0.000368
    • LIPO(Sec/SPII): 0.00035
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNERHADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSWCKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQNYTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRPCQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLTSFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDKSLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQYFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKALKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIKYYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNFSTDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLIQAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFMDAYRELKMIVPTPHMVDDIQDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARKAIESFVAETTEKYGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=JSNZ_000138&oldid=105779"