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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001364
  • pan locus tag?: SAUPAN003786000
  • symbol: nikC
  • pan gene symbol?: nikC
  • synonym: opp2C
  • product: nickel transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001364
  • symbol: nikC
  • product: nickel transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1378972..1379802
  • length: 831
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCATAAAATATATTCAAAGAATAACCTGATATTTTTTGTATTCGTTGCATTTATTTTT
    GTGGTAATTGTACTGCAATTCTTTGTCAGTAGTGAAAATGCAACCAAAGTCAATTTATCA
    CAAACTTTTGAACCTATTAGTTGGTTGCATTTATTAGGAACTGATGATTATGGGAGAGAT
    TTATTTACCCGAATTATTATCGGTGCACGTTCAACATTGTTTGTTACTGTTTTAACATTA
    ATAGCTATCGTTGTCATAGGTGTTACACTAGGTCTATTTGCCGGATATAAAAAAGGGTGG
    ATTGAACGATTAGTGTTAAGGTTTATTGATGTTGGTCTAAGTATTCCAGAATTTATCATC
    ATGATTGCTTTAGCAAGCTTTTTTCAACCATCTTTATGGAATTTAGTTATCTCAATTACA
    GTAATAAAATGGATGAATTACACAAGGTTGACTAGAAGTATAGTTAATAGCGAAATGAAT
    AAGCCCTATATAAAAATGGCACAATTATTTCATGTACCTACAAGAACAATATTAATACGT
    CATTTAACACCTAAAATTATACCGGCTATTATCGTTTTGATGGTCGTTGATTTCGGTAAA
    ATCATTCTATATATAAGTTCACTATCATTTATTGGGTTAGGTGCACAACCGCCAACACCA
    GAGTGGGGCGCTATGTTGCAACAAGGTCGTGATTTTATTTCGTCTCATCCAATTATGTTG
    ATTGCACCTGCTTCAGTCATTGCTATAACTATTTTAATTTTTAATTTAACCGGTGATGCA
    CTAAGAGATAGATTGCTGAAACAACGGGGTGAATATGATGAGTCTCATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001364
  • symbol: NikC
  • description: nickel transporter permease
  • length: 276
  • theoretical pI: 10.1818
  • theoretical MW: 31257.1
  • GRAVY: 0.673913

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 246.9)
    and 2 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 22)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 19.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 101)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.370353
    • TAT(Tat/SPI): 0.001476
    • LIPO(Sec/SPII): 0.054876
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MHKIYSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFEPISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIERLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMNKPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPPTPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Brunella Posteraro, Marie Carrière, Laetitia Remy, Cécile Delporte, Marilena La Sorda, Maurizio Sanguinetti, Vincent Juillard, Elise Borezée-Durant
A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection.
Mol Microbiol: 2010, 77(5);1246-60
[PubMed:20662775] [WorldCat.org] [DOI] (I p)

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