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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001972
  • pan locus tag?: SAUPAN005004000
  • symbol: JSNZ_001972
  • pan gene symbol?: aspB
  • synonym:
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001972
  • symbol: JSNZ_001972
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • replicon: chromosome
  • strand: +
  • coordinates: 1983267..1984553
  • length: 1287
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATCCTTTAGCCCAAAGCTTAAATGAACAACTTCAACAGTCAAATGCAACTGCCTTT
    GCAATGTTATCTGACTTAGGTCAAAATATGTTTTATCCAAAAGGCATTTTATCTCAATCT
    GCTGAAGCAAAGAGTACAACATATAATGCAACTATAGGTATGGCGACAAACAAAGACGGA
    AAAATGTTTGCATCATCTTTAGATGCAATGTTTAATAATTTAACTCCAGATGAAATATTC
    CCTTATGCGCCACCACAAGGCATCGAAGAATTACGTGATTTATGGCAACAAAAAATGTTG
    CGTGACAATCCAGAGCTATCAATCGACAACATGTCACTACCAATTGTTACGAATGCATTA
    ACACATGGTTTATCTTTAGTTGGCGATTTATTTGTAAATCAAGGTGACACTATCTTGTTA
    CCAGATCATAATTGGGGTAATTACAAACTTGTTTTCAATACGAGAAATGGTGCAAACCTT
    CAAATATATCCTATCTTTGATAAAGACGGGCATTATACTACTGATTCACTTGTAGAAGCT
    TTACAATCATACAATAAAGATAAAGTCATTATGATTTTAAATTATCCTAATAATCCGACA
    GGTTACACACCTACGCATAAAGAAGTGACTACCATCGTCGATGCAATTAAAGCATTAGCT
    GATAAAGGTACAAAAGTTATAGCTGTTGTGGATGACGCATACTATGGTTTATTCTATGAA
    GATGTGTATACTCAATCATTATTTACTGCATTATCTAATTTAAATTCAAATGCAATATTA
    CCTGTTCGTTTAGATGGTGCAACAAAAGAATTTTTCGCATGGGGATTCCGTGTTGGGTTT
    ATGACATTTGGAACGTCTGATCAAACAACTAAAGAAGTATTAGAAGCCAAAGTAAAAGGT
    CTTATACGAAGTAACATTTCTAGTGGACCTCTTCCAACACAAAGCGCTGTTAAGCATTTA
    TTAAAAAATAATAAACAATTCGATAAAGAAATCGAACAAAATATTCAAACATTAAAAGAA
    CGCTATGAAGTCACTAAGGAAGTTGTCTATGCTGATCAATATCATTCACATTGGCAAGCC
    TATGACTTTAACTCTGGATACTTTATGGCAATAAAAGTTCATGATGTTGACCCTGAAGAA
    CTTCGTAAACATTTAATTGAAAAATATTCAATTGGTGTTATTGCACTTAATGCAACAGAT
    ATTCGTATTGCGTTTAGCTGTGTAGAAAAAGAAGATATCCCACATGTATTTGATTCAATT
    GCTAAAGCGATTGATGACTTAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001972
  • symbol: JSNZ_001972
  • description: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • length: 428
  • theoretical pI: 4.95236
  • theoretical MW: 48100.1
  • GRAVY: -0.299299

⊟Function[edit | edit source]

  • TIGRFAM:
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 62.8)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 62.1)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 56.5)
    and 9 more
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 40.8)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 33.2)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 31.8)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 26.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 25.4)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 22.7)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 19)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.1)
    [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 10.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 125.6)
    and 4 more
    no clan defined NEMO; NF-kappa-B essential modulator NEMO (PF11577; HMM-score: 14.7)
    Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 14.2)
    4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 13.8)
    no clan defined Mst1_SARAH; C terminal SARAH domain of Mst1 (PF11629; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9871
    • Cytoplasmic Membrane Score: 0.0012
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0113
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02551
    • TAT(Tat/SPI): 0.013368
    • LIPO(Sec/SPII): 0.00165
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAKSTTYNATIGMATNKDGKMFASSLDAMFNNLTPDEIFPYAPPQGIEELRDLWQQKMLRDNPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPDHNWGNYKLVFNTRNGANLQIYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKALADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGFRVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHLLKNNKQFDKEIEQNIQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIGVIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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