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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002299
  • pan locus tag?: SAUPAN005818000
  • symbol: hutU
  • pan gene symbol?: hutU
  • synonym:
  • product: urocanate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002299
  • symbol: hutU
  • product: urocanate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2296120..2297781
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAAAAATTCAAGCAAAAAAAGGTTTGAGTATTGAGTGTAAAGGTTGGGAACAAGAA
    GCAGTACTTAGAATGTTATACAACAATTTAGACCCAGAAGTTGCTGAAAGACCAGAAGAT
    TTAGTTGTTTATGGTGGTATTGGAAAAGCAGCACGTAATTGGGAAGCTTTTGAAGCAATA
    GAGAAAACGTTACGAGAATTAGAATCAGATGAGACAATGTTAGTACAATCAGGAAAGCCA
    GTTGCTGTATTTAAAACACATGAAGAAGCGCCACGTGTATTAATTTCAAATTCAGTATTA
    GTGCCTGAATGGGCAAACTGGGATCATTTTAATGAATTAGATAAAAAAGGTTTAATCATG
    TATGGACAGATGACAGCCGGTAGTTGGATATATATTGGTTCTCAAGGTATTGTGCAAGGT
    ACTTATGAGACATTTGCCGAGCTAGGTAATCAACACTTTAATGGAGATTTAGCAGGCACT
    GTTACACTGACAGCAGGTTTAGGTGGTATGGGTGGTGCGCAACCGTTAGCAATTACTATG
    AATCATGGGGTAGCAATTTGCGTGGATGTTGATGAAACACGTGTTGATAAGCGAATTGAT
    ACGAAATACTGTGATGTTAAAACAGCTGATTTAGATGAAGCATTAAAATTAGCAGAAGAG
    GCGAAAGAACGTGGAGAAGGATTATCAATCGGGTTAGTTGGAAATGCTGTAGACATCCAT
    CAAGCGATTCTAGAAAAAGGATTTAAAATTGACATTATTACTGACCAAACAAGTGCACAT
    GATCCGCTAAATGGATATGTGCCACAAGGATACTCTGTAGAAGAAGCGAAAGTATTGCGT
    GAAAAAGATCCGAAAAAATATGTTGAACTGTCACAAGCTTCAATGGCAAAGCATGTTGAA
    TTAATGCTTGAATTCCAAAAACGTGGAGCTGTAGCATTTGATTATGGTAACAATATTCGT
    CAAGTAGCCTTCAATAACGGAGTGAAAAATGCTTTTGACTTCCCAGGCTTTGTACCAGCT
    TACATTAGACCATTATTCTGTGAAGGTAAAGGGCCATTCCGCTTTGCTGCGTTGAGTGGT
    GATCCAAAAGATATCGAGCGTGCGGATGAAGAAATGCGCAAACTTTTCCCAGAAAATGAA
    AAGTTATTAAGATGGCTTGATTTAGCTGAAGAAAAAATTTCATATCAAGGACTACCATCA
    CGTATTGCTTGGTTAGGCTATGGAGAAAGAGCGAAAATGGGCTTAGCTTTAAATCGTCTT
    GTACGTGACGGTGAAATTTCAGCACCAATTGTTATTGGACGAGACCATTTAGATGCTGGT
    TCAGTTGCTAGTCCTAACCGTGAAACAGAAAGTATGAAAGATGGTAGTGACGCGGTTGGT
    GATTGGGCTGTATTGAATGCACTCATTAACACAGCTGCAGGTGGTTCATGGATTTCATTC
    CATCACGGTGGCGGTGTTGGCATGGGATATTCACTTCATGCGGGTATGGTTGTTGTAGCA
    GATGGATCAGAGCGTGCTGAAAGAAGATTGGAACGTGTATTGACGACTGACCCAGGTATG
    GGTGTTGCCCGACATGTTGATGCTGGCTATGACATCGCTATTCAAACAGCTAAAGAAAAA
    GGTATTCATATTCCAATGATTGATAAAGCAGGTGATAAGTAA
    60
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    720
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    840
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    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002299
  • symbol: HutU
  • description: urocanate hydratase
  • length: 553
  • theoretical pI: 5.03205
  • theoretical MW: 60632.4
  • GRAVY: -0.292586

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.49?  ExPASy
    Urocanate hydratase 3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines urocanate hydratase (TIGR01228; EC 4.2.1.49; HMM-score: 969.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Urocanase; Urocanase Rossmann-like domain (PF01175; HMM-score: 314.5)
    no clan defined Urocanase_C; Urocanase C-terminal domain (PF17392; HMM-score: 298.2)
    and 3 more
    Urocanase_N; Urocanase N-terminal domain (PF17391; HMM-score: 202.8)
    DUF6429; Domain of unknown function (DUF6429) (PF20008; HMM-score: 13.6)
    Exonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.946
    • Cytoplasmic Membrane Score: 0.0009
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.053
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00465
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.00071
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLAEEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HutR* (activation) regulon, CcpA regulon
    HutR*(TF)important in Histidine utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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