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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2324 [new locus tag: SACOL_RS12220 ]
  • pan locus tag?: SAUPAN005818000
  • symbol: hutU
  • pan gene symbol?: hutU
  • synonym:
  • product: urocanate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2324 [new locus tag: SACOL_RS12220 ]
  • symbol: hutU
  • product: urocanate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2385739..2387400
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAAAAATTCAAGCAAAAAAAGGTTTGAGTATTGAGTGTAAAGGTTGGGAACAAGAA
    GCAGTACTTAGAATGTTATACAACAATTTAGACCCAGAAGTTGCTGAAAGACCAGAAGAT
    TTAGTTGTTTATGGTGGTATTGGAAAAGCAGCACGTAATTGGGAAGCTTTTGAAGCAATA
    GAGAAAACGTTACGAGAATTAGAATCAGATGAGACAATGTTAGTACAATCAGGAAAGCCA
    GTTGCTGTATTTAAAACACATGAAGAAGCGCCACGTGTATTAATTTCAAATTCAGTATTA
    GTGCCTGAATGGGCAAACTGGGATCATTTTAATGAATTAGATAAAAAAGGTTTAATCATG
    TATGGACAGATGACAGCCGGTAGTTGGATATATATTGGTTCTCAAGGTATTGTGCAAGGT
    ACTTATGAGACATTTGCCGAGCTAGGTAATCAACACTTTAATGGAGATTTAGCAGGCACT
    GTTACACTGACAGCAGGTTTAGGTGGTATGGGTGGTGCGCAACCGTTAGCAATTACTATG
    AATCATGGGGTAGCAATTTGCGTGGATGTTGATGAAACACGTGTTGATAAGCGAATTGAT
    ACGAAATACTGTGATGTTAAAACAGCTGATTTAGATGAAGCATTAAAATTAGCAGAAGAG
    GCGAAAGAACGTGGAGAAGGATTATCAATCGGGTTAGTTGGAAATGCTGTAGACATCCAT
    CAAGCGATTCTAGAAAAAGGATTTAAAATTGACATTATTACTGACCAAACAAGTGCACAT
    GATCCGCTAAATGGATATGTGCCACAAGGATACTCTGTAGAAGAAGCGAAAGTATTGCGT
    GAAAAAGATCCGAAAAAATATGTTGAACTGTCACAAGCTTCAATGGCAAAGCATGTTGAA
    TTAATGCTTGAATTCCAAAAACGTGGCGCTGTAGCATTTGATTATGGTAACAATATTCGT
    CAAGTAGCCTTCAATAACGGAGTGAAAAATGCTTTTGACTTCCCAGGCTTTGTACCAGCT
    TACATTAGACCATTATTCTGTGAAGGTAAAGGGCCATTCCGCTTTGCTGCGTTGAGTGGT
    GATCCAAAAGATATCGAGCGTGCGGATGAAGAAATGCGCAAACTTTTCCCAGAAAATGAA
    AAGTTATTAAGATGGCTTGATTTAGCTGAAGAAAAAATTTCATATCAAGGACTACCATCA
    CGTATTGCTTGGTTAGGCTATGGAGAAAGAGCGAAAATGGGCTTAGCTTTAAATCGTCTT
    GTACGTGACGGTGAAATTTCAGCACCAATTGTTATTGGACGAGACCATTTAGATGCTGGT
    TCAGTTGCTAGTCCTAACCGTGAAACAGAAAGTATGAAAGATGGTAGTGACGCGGTTGGT
    GATTGGGCTGTATTGAATGCACTCATTAACACAGCTGCAGGTGGTTCATGGATTTCATTC
    CATCACGGTGGCGGTGTTGGCATGGGATATTCACTTCATGCGGGTATGGTTGTTGTAGCA
    GATGGATCAGAGCGTGCTGAAAGAAGATTGGAACGTGTATTGACGACTGACCCAGGTATG
    GGTGTTGCCCGACATGTTGATGCTGGCTATGACATCGCTATTCAAACAGCTAAAGAAAAA
    GGTATTCATATTCCAATGATTGATAAAGCAGGTGATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2324 [new locus tag: SACOL_RS12220 ]
  • symbol: HutU
  • description: urocanate hydratase
  • length: 553
  • theoretical pI: 5.03205
  • theoretical MW: 60632.4
  • GRAVY: -0.292586

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.49?  ExPASy
    Urocanate hydratase 3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines urocanate hydratase (TIGR01228; EC 4.2.1.49; HMM-score: 969.4)
  • TheSEED  :
    • Urocanate hydratase (EC 4.2.1.49)
    Amino Acids and Derivatives Histidine Metabolism Histidine Degradation  Urocanate hydratase (EC 4.2.1.49)
  • PFAM:
    NADP_Rossmann (CL0063) Urocanase; Urocanase Rossmann-like domain (PF01175; HMM-score: 302.8)
    no clan defined Urocanase_C; Urocanase C-terminal domain (PF17392; HMM-score: 299)
    and 3 more
    Urocanase_N; Urocanase N-terminal domain (PF17391; HMM-score: 203.8)
    TPR (CL0020) TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 12.3)
    no clan defined Exonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: NAD+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00465
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.00071
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLAEEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HutR* (activation) regulon, CcpA regulon
    HutR*(TF)important in Histidine utilization; RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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