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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002619
  • pan locus tag?: SAUPAN006357000
  • symbol: clfB
  • pan gene symbol?: clfB
  • synonym:
  • product: MSCRAMM family adhesin clumping factor ClfB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002619
  • symbol: clfB
  • product: MSCRAMM family adhesin clumping factor ClfB
  • replicon: chromosome
  • strand: -
  • coordinates: 2622946..2625333
  • length: 2388
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    TTGAAAAAAAGAATTGATTATTTGTCGAATAAGCAGAATAAGTATTCGATTAGACGTTTT
    ACAGTAGGTACCACATCAGTAATAGTAGGGGCAACTATACTATTTGGGATAGGCAATCAT
    CAAGCACAAGCTTCAGAACAATCGAACGATACAACGCAATCTTCGAAAAATAATGCAAGT
    GCAGATTCCGAAAAAAACAATATGATAGAAACACCTCAATTAAATACAACGGCTAATGAT
    ACATCTGATATTAGTGCAAACACAAACAGTGCGAATGTAGATAGCACAGCAAAACCAATG
    TCTACACAAACGAGCAATACCACTACAACAGAGCCAGCTTCAACAAATGAAACACCTCAA
    CCGACGGCAATTAAAGATCAAGCAACTGCTGCAAAAATGCAAGATCAAACTGTTCCTCAA
    GAAGCAAATTCTCAAGTAGATAATAAAACAACGAATGATGCTAATAGCATAGCGACAAAC
    AGTGAGCTTAAAAATCCTCAAACATTAGATTTACCACAATCATCACCACAAACGATTTCC
    AATGCGCAAAGAACTAGTAAACCAAGTGTTAGAACGAGAGCTGTACGTAGTTTAGCTGTT
    GCTGAACCGGTAGTAAATGCTGCTGATGCTAAAGGTACAAATGTAAATGGTCAAGTTACG
    GCAAGTGATTTCAAGTTAGAAAAGACTACATTTGACCCTAACCAAAGTGGTAACACATTT
    ATGGCGGTAAATTTTAAAGTGGCAGGGAAAGTGAAATCAGGGGATTATTATACAGCGAAG
    TTACCAGATAGTTTAACTGGTAATGGAGACGTGGATTACTCTAATTCAAATAATACGATG
    CCAATTGCAGATATTAAAAGTACGAATGGCGATGTTGTAGCTAAAGCAACATATGATATC
    TTGACTAAGACGTATACATTTGTCTTTACAGATTATGTAAATGATAAAGAAAATATTAAC
    GGACAATTTTCATTACCTTTATTTACAGACCGAGCAAAGGCACCTAAATCAGGAACATAC
    GATGCAAATATTAATATTGCGGATGAAATGTTTAATAATAAAATTACTTATAACTATAGT
    TCGCCAATTGCAGGAATTGATAAGCCAAATGGCGCGAACATTTCTTCTCAAATTATTGGT
    GTAGATACAGCTTCAGGTCAAAACACATACAAGCAAACGGTATTTGTTAACCCTAAGCAA
    CGAGTTTTAGGTAATACGTGGGTGTATATTAAAGGTTACCAAGATAAAATCGAAGAAAGT
    AGCGGTAAAGTAAGTGCTACAGATACAAAACTGAGAATTTTTGAAGTGAATGATACATCT
    AAATTATCAGATAGCTACTATGCAGATCCAAATGACTCTAACCTTAAAGAAGTAACGAAT
    GAGTTTAAGGATAAAATCACTTATAAATACCAAAATGTAGCAAGTATTAATTTTGGCGAT
    ATTACTAAAACGTATGTTGTATTAGTGGAAGGTCACTATGATAATACTGGTAAAAACTTG
    AAAACACAGGTTATTCAAGAAAATATTGACCCAGCGACAGGTAAAGACTACAGTATTTTC
    GGTTGGAATAATGAGAATGTTGTACGTTATGGTGGTGGAAGTGCTGATGGTGATTCAGCA
    GTAAATCCGAAAGACCCAACTCCAGGGCCACCGGTTGACCCAGAACCAAGTCCAGATCCA
    GAACCGGAGCCAACGCCAGATCCAGAACCAAGTCCAGACCCAGATCCGGATTCAGATTCA
    GACAGTGACTCAGGCTCAGACAGCGACTCAGGTTCAGATAGCGATTCGGATTCAGATAGC
    GACTCAGAATCAGACAGTGATTCAGAATCAGACAGCGATTCAGACTCAGACAGTGAGTCA
    GATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGAATCAGATAGCGACTCAGAATCA
    GACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGC
    GATTCAGATTCAGATAGCGACTCAGACAGCGATTCAGAATCAGACAGCGACTCAGACTCA
    GATAGCGATTCAGACTCAGACAGTGATTCAGAATCAGACAGCGACTCAGACTCAGATAGC
    GATTCAGATTCAGATAGCGACTCGGATTCAGATAGCGATTCGGACTCAGATAGTGACTCC
    GATTCAAGAGTTACACCACCAAATAATGAACAGAAAGCACCATCAAATCCTAAAGGTGAA
    GTAAACCATTCTAATAAGGTATCAAAACAACACAAAACTGATGCTTTACCAGAAACAGGA
    GATAAGAGCGAAAACACAAATGCAACTTTATTTGGTGCAATGATGGCATTATTAGGATCA
    TTACTATTGCTTAGAAAACGCAAGCAAGATCATAAAGAAAAAGCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2388

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002619
  • symbol: ClfB
  • description: MSCRAMM family adhesin clumping factor ClfB
  • length: 795
  • theoretical pI: 3.92086
  • theoretical MW: 85133.9
  • GRAVY: -1.06075

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 52.2)
    and 2 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 28.4)
    pullulanase, extracellular (TIGR02102; HMM-score: 18.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 138.3)
    and 6 more
    E-set (CL0159) Big_8; Bacterial Ig domain (PF17961; HMM-score: 82.5)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44.2)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 38.4)
    GBD (CL0202) Androglobin_IV; Androglobin, domain IV (PF22069; HMM-score: 18.9)
    no clan defined T3SS_CopN_3rd; T3SS, Low calcium response E, third helical domain (PF22342; HMM-score: 14.6)
    LRR (CL0022) DUF3439; Domain of unknown function (DUF3439) (PF11921; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0107
    • Cell wall & surface Score: 0.8786
    • Extracellular Score: 0.1106
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 44 aa
    • SP(Sec/SPI): 0.899149
    • TAT(Tat/SPI): 0.06812
    • LIPO(Sec/SPII): 0.003061
    • Cleavage Site: CS pos: 44-45. AQA-SE. Pr: 0.9420
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQASEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTAKPMSTQTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANSIATNSELKNPQTLDLPQSSPQTISNAQRTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNGQVTASDFKLEKTTFDPNQSGNTFMAVNFKVAGKVKSGDYYTAKLPDSLTGNGDVDYSNSNNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKAPKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTNEFKDKITYKYQNVASINFGDITKTYVVLVEGHYDNTGKNLKTQVIQENIDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPTPDPEPSPDPDPDSDSDSDSGSDSDSGSDSDSDSDSDSESDSDSESDSDSDSDSESDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLLRKRKQDHKEKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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