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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002677
  • pan locus tag?: SAUPAN006445000
  • symbol: JSNZ_002677
  • pan gene symbol?: drp35
  • synonym:
  • product: SMP-30/gluconolactonase/LRE family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002677
  • symbol: JSNZ_002677
  • product: SMP-30/gluconolactonase/LRE family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2695698..2696669
  • length: 972
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCACAACAAGATTTACCTACATTATTTTATAGCGGGAAGTCCAATAGTGCTGTTCCA
    ATTATATCTGAAAGTGAATTACAAACAATTACAGCTGAACCATGGCTTGAAATTTCCAAA
    AAAGGATTGCAACTAGAAGGATTGAACTTTGATCGGCAGGGACAACTCTTTTTATTGGAT
    GTATTCGAAGGCAATATTTTCAAAATCAATCCTGAAACGAAGGAAATCAAACAACCTTTT
    GTAAGTCACAAAGCGAATCCTGCAGCAATCAAAATACATAAAGATGGCCGATTATTCGTT
    TGTTATTTAGGAGATTTTAAATCTACAGGAGGCATTTTTGCAGCTACAGAAAATGGTGAC
    AACTTACAAGATATTATTGAAGATCTTTCAACAGCATATTGTATTGATGACATGGTATTT
    GATTCTAAAGGTGGATTTTATTTTACAGATTTTAGAGGATACTCTACCAATCCACTAGGA
    GGCGTTTATTATGTTTCGCCGGACTTTAGAACAGTGACGCCTATCATTCAAAATATTAGC
    GTAGCAAATGGTATTGCTTTAAGTACAGATGAAAAAGTACTATGGGTAACAGAAACTACA
    GCCAATCGATTACATCGCATTGCACTTGAAGATGATGGTGTGACGATACAACCATTTGGA
    GCTACTATACCGTACTATTTTACAGGTCATGAAGGACCAGACTCATGTTGTATTGATAGT
    GACGATAATTTATACGTAGCAATGTATGGTCAAGGTCGAGTGTTAGTTTTTAATAAAAGG
    GGTTATCCAATAGGACAAATATTGATACCAGGCCGAGATGAAGGGCATATGTTACGTTCT
    ACTCATCCGCAATTTATACCTGGAACAAATCAACTCATCATTTGTTCCAATGATATAGAA
    ATGGGCGGAGGATCTATGCTTTATACAGTTAATGGCTTTGCAAAAGGTCATCAAAGTTTT
    CAGTTTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002677
  • symbol: JSNZ_002677
  • description: SMP-30/gluconolactonase/LRE family protein
  • length: 323
  • theoretical pI: 4.63671
  • theoretical MW: 35804.1
  • GRAVY: -0.189474

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Beta_propeller (CL0186) SGL; SMP-30/Gluconolactonase/LRE-like region (PF08450; HMM-score: 102.1)
    and 5 more
    Arylesterase; Arylesterase (PF01731; HMM-score: 28.9)
    Str_synth; Strictosidine synthase (PF03088; HMM-score: 27.8)
    NHL; NHL repeat (PF01436; HMM-score: 24.3)
    Vgb_lyase; Virginiamycin B lyase family (PF24684; HMM-score: 18.3)
    Mala_s_1-like; Mal s 1 allergenic protein-like (PF22701; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9471
    • Cytoplasmic Membrane Score: 0.0292
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0234
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003495
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.000415
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQLEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKQPFVSHKANPAAIKIHKDGRLFVCYLGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLGGVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQPFGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGHMLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, VraR (activation) regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    VraR(TF)response regulator important for resistance against cell-wall targeting antibiotics;  regulation predicted or transferred from N315 and NCTC 8325  [2] [3]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)
  2. ↑ Makoto Kuroda, Hiroko Kuroda, Taku Oshima, Fumihiko Takeuchi, Hirotada Mori, Keiichi Hiramatsu
    Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus.
    Mol Microbiol: 2003, 49(3);807-21
    [PubMed:12864861] [WorldCat.org] [DOI] (P p)
  3. ↑ Susan Boyle-Vavra, Shouhui Yin, Dae Sun Jo, Christopher P Montgomery, Robert S Daum
    VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.
    Antimicrob Agents Chemother: 2013, 57(1);83-95
    [PubMed:23070169] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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