PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0026 [new locus tag: NWMN_RS00160 ]
• pan locus tag^?: SAUPAN000489000
• symbol: NWMN_0026
• pan gene symbol^?: tauE
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0026 [new locus tag: NWMN_RS00160 ]
• symbol: NWMN_0026
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 37219..37974
• length: 756
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329931 NCBI
• RefSeq: YP_001331061 NCBI
• BioCyc:
• MicrobesOnline: 3705557 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGATATTGTTAATATAGTTATCATGTTACTCATTGGGGTATTCGGTGGGTTCATATCT
  GGTCTAGTAGGCGTAGGTGGTGCTATCATTATTTATCCAGCTATTTTATTATTACCACCA
  TTATTCGGAGCCCCTGCATATAGCGCATATATCGCATCAGGTCTTACTTCTAGCCAAGTC
  TTTTTCAGTACATTAAGTGGCTCATTAAAAGCTCGAAAAAAAACAGAGTTCTCGCCACAA
  TTAGTTCTTTATATGGGTGGCGGCATGATTATAGGGAGTATGTTAGGTGCATTTCTAGCA
  AATTTATTTGATGCTACATTTGTAAATACGGTATATATCATTATTGCTTTACTTGCATTA
  ACATTAATGTTTATTAAAGTGAAACCTAGTTCCGAAAAGTCTTCATTTAATAAATATTTA
  TTAGTTATCATTGGCCTTTTCATTGGTATTATTTCAGGTATAGTAGGTGCAGGTGGTGCC
  TTCATTATTATTCCTATATTATTGGTTTTATTCAAATTACCAATGAATACTGTAGTCGCT
  AATAGTATAGTAATTGCTTTTATATCTTCAATTGGTGCTTTTGTCATCAAACTTATTCAA
  GGTTATATCCCTTTATATGACGCCTTATTCCTTATAATAGGGAGTATTATATTTGCTCCA
  ATAGGGTTAAAATTAAGTAAAAAAGTCCCTAACATAATACAAAAATGGATTATCAGTATT
  TTAATCGTTTTTGCTATAGTTCAACTCATTTTATAA
  60
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  756
  

⊟Protein

⊟General

• locus tag: NWMN_0026 [new locus tag: NWMN_RS00160 ]
• symbol: NWMN_0026
• description: hypothetical protein
• length: 251
• theoretical pI: 10.2985
• theoretical MW: 26690.6
• GRAVY: 1.3992

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  LysE (CL0292) TauE; Sulfite exporter TauE/SafE (PF01925; HMM-score: 140.8)
  and 1 more
  no clan defined DUF3488; Domain of unknown function (DUF3488) (PF11992; HMM-score: 6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.054892
  • TAT(Tat/SPI): 0.009404
  • LIPO(Sec/SPII): 0.016165
• predicted transmembrane helices (TMHMM): 7

⊟Accession numbers

• GI: 151220238 NCBI
• RefSeq: YP_001331061 NCBI
• UniProt: A0A0H3K5H7 UniProt

⊟Protein sequence

• MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGAPAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANLFDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGAFIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIFAPIGLKLSKKVPNIIQKWIISILIVFAIVQLIL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Jiangchuan Shen, Mary E Keithly, Richard N Armstrong, Khadine A Higgins, Katherine A Edmonds, David P Giedroc
Staphylococcus aureus CstB Is a Novel Multidomain Persulfide Dioxygenase-Sulfurtransferase Involved in Hydrogen Sulfide Detoxification.
Biochemistry: 2015, 54(29);4542-54
[PubMed:26177047] [WorldCat.org] [DOI] (I p)