PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0027 [new locus tag: NWMN_RS00170 ]
• pan locus tag^?: SAUPAN000487000
• symbol: NWMN_0027
• pan gene symbol^?: cstA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0027 [new locus tag: NWMN_RS00170 ]
• symbol: NWMN_0027
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 38371..39438
• length: 1068
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329932 NCBI
• RefSeq: YP_001331062 NCBI
• BioCyc:
• MicrobesOnline: 3705558 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAACAATACGGAGAAAAGTTTATCGATGAATTTAGTAAAGCAGAATTGGAAAAACTA
  GCCAAGCAAGGGCAATTAATTGACGTTAGAACAGAAGAGGAGTATGCATTAGGACATATC
  AATGGTTCCATACTTCATCCTGTTGATGAGATTGAGTCATTCAATAAAGAAAAAAATAAA
  ACCTATTATGTAATCTGTAGAAGTGGTAACAGAAGTGCTAATGCTAGTAAATATTTAGCT
  AAACAAGGTTATAACGTTATAAATCTTGATGGTGGTTATAAAGCTTATGAAGAAGAAAAC
  GATAGTTATGATACACAAGAAGAATATAAAAGTATAGAAATTAAAGCAGATCGTAAACAA
  TTTAACTATCGTGGTCTTCAATGTCCAGGGCCAATTGTAAAAATTAGTCAAGAAATGAAG
  AATATTGAAGTAGGTGACCAAATTGAAGTCAAAGTCACAGACCCTGGATTCCCTAGTGAC
  ATTAAAAGTTGGGTGAAACAAACAAGGCATACTTTAGTTAAGCTTGATGAAAATAACAAT
  GGAATTAATGCGATTATTCAAAAAGAAAAAGCAAAAGATTTAGATATAAATTATTCTGCT
  AAAGGTACTACAATTGTATTATTTAGTGGAGAATTAGACAAAGCTGTAGCAGCGTTGATT
  ATTGCAAATGGTGCTAGAGCTGCTGGAAAAGATGTAACTATCTTCTTTACTTTTTGGGGG
  CTTAATGCATTAAAAAAAGTGCAAACAGTTAATGTTAAAAAGCAAGGTATTGCAAAAATG
  TTTGATTTAATGTTGCCCAAAAAGAATATACGAATGCCTCTTTCCAAAATGAATATGTTT
  GGTTTAGGAAATATGATGATGCGCTACGTAATGAAAAAGAAAAATGTTGATTCATTACCA
  ACACTTATCAATCAAGCTATTGAGCAAAATATCAAATTAATCGCTTGTACGATGAGTATG
  GATGTCATGGGTATTCAGAAAGAAGAACTTAGAGATGAAGTTGAGTACGGTGGTGTAGGC
  ACTTATATTGGTGCTACTGAAAATGCGAATCATAATTTATTTATCTAA
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⊟Protein

⊟General

• locus tag: NWMN_0027 [new locus tag: NWMN_RS00170 ]
• symbol: NWMN_0027
• description: hypothetical protein
• length: 355
• theoretical pI: 8.36646
• theoretical MW: 40086.8
• GRAVY: -0.448169

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 36.7)
  phage shock operon rhodanese PspE (TIGR02981; EC 2.8.1.1; HMM-score: 31.4)
  and 2 more
  selenium metabolism protein YedF (TIGR03527; HMM-score: 23.6)
  thiazole biosynthesis domain (TIGR04271; HMM-score: 12.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  DsrEFH-like (CL0394) DrsE_2; DsrE/DsrF/DrsH-like family (PF13686; HMM-score: 195.4)
  and 4 more
  TusA-like (CL0397) TusA; Sulfurtransferase TusA (PF01206; HMM-score: 70.8)
  Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 59.4)
  DsrEFH-like (CL0394) DrsE; DsrE/DsrF-like family (PF02635; HMM-score: 40.5)
  6_Hairpin (CL0059) Prenyltrans; Prenyltransferase and squalene oxidase repeat (PF00432; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005604
  • TAT(Tat/SPI): 0.001709
  • LIPO(Sec/SPII): 0.001465
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220239 NCBI
• RefSeq: YP_001331062 NCBI
• UniProt: A0A0H3K610 UniProt

⊟Protein sequence

• MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDSYDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0027 > NWMN_0028 > NWMN_0029

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Jiangchuan Shen, Mary E Keithly, Richard N Armstrong, Khadine A Higgins, Katherine A Edmonds, David P Giedroc
Staphylococcus aureus CstB Is a Novel Multidomain Persulfide Dioxygenase-Sulfurtransferase Involved in Hydrogen Sulfide Detoxification.
Biochemistry: 2015, 54(29);4542-54
[PubMed:26177047] [WorldCat.org] [DOI] (I p)