PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
• pan locus tag^?: SAUPAN000385000
• symbol: NWMN_0032
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
• symbol: NWMN_0032
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 44597..45817
• length: 1221
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329937 NCBI
• RefSeq: YP_001331067 NCBI
• BioCyc:
• MicrobesOnline: 3705563 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGACTGATTTGTTGAAAATTAAAAACTTCAGATTGTTCTTTTTAGCAGAAATAATTTCA
  GCATTCGGAGTTGGAATTAGTACAGTTGGGGCTAATTGGTATTTAATCGACAAAACTAAC
  GACTCCCAACTATTAGGTATTATGTTAGCTTTAAATGTATTATCCGGTTTCTTAGCTTCT
  CCTATAATAGGTGGACTAGCCGATAAATATAATAGACGAAATATTATTTTAATCACCTAT
  CTTCTTCAGGTAATATTATATTTACTCATTGTCATAGCACTCGTAATGATAGGATTTGAG
  ACCTATTTAGTGATTGGATTTGCGATTGTTAATGGGATAGGTTGGACTACGTATATGGCA
  ACATCTAGAAGTTTAGTTAAACAGATATTAAAACCAGATCAATATACAGATGCAAATTCT
  CTATTAGAGATTAGTTTGCAAACAGGTATGTTTATTGCAGGTGGATTATCAGGAATATTA
  TATAAAATAAATGGATTCACTCTAATTATAGCGATGACTATAATGATGTTTCTAATCAGC
  ATTTTTATGTTATTTAGATTGCATGTAGATAAACCAACTCATTCAGAGGAAGAATCAACA
  AATAGCTTATTGCAAGAATATTTATTAGGATGGAAATTTTTAAAAGACAACATGATGATA
  TTTATTTTTGGAGTTATTTCAATCATACCAATGGTGTTTACAATGATCTTTAACATATCA
  TTACCAGGATATGTTTATAATGTTTTAAAACTTTCGTCTGTACAATTTGGTTTTTCAGAT
  ATGTTATATGGCATTGGAGGATTATGTGCAGGTTTAATTTCAGCTATTCTTTCGAAGAAA
  ATCTCAACTAAAGTATTGATATTTTTGTTATATTTCATATTAGTCATAAATTCAGCACTA
  TTTATTTGGATAAACTCAGCATTTTACTTATTCATAGGATCATTTATACTAGGCTACTCA
  ATTTCATCAATCAGAATTTATATGAATACAGCTATAATGAACACTGTTTCAGATAAATAT
  GTCGGTCGCTCATTTACGATATGGACGTCAATTTCATTGTTACTGCAAAGTTTAATTGCT
  CCATTTTTAGGAAGATGGATTAATGAAATTAATGATAAATTCGGTTTCTATATTATACTC
  ATTTTATCCTTACTCATATTTGTTACACTGCTGCTTGTTAACAAAACAGACAAAATAAAA
  TATGCACATAAAGAAGAGTGA
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⊟Protein

⊟General

• locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
• symbol: NWMN_0032
• description: hypothetical protein
• length: 406
• theoretical pI: 9.43757
• theoretical MW: 45716.5
• GRAVY: 0.86798

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 165.1)
  and 8 more
  multidrug resistance protein (TIGR00880; HMM-score: 67.8)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 26.6)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 16.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 12.6)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 8.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar (Glycoside-Pentoside-Hexuronide) transporter (TIGR00792; HMM-score: 7.9)
• TheSEED: data available for COL, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 109)
  and 4 more
  MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 60)
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 30.5)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.7)
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 9.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024105
  • TAT(Tat/SPI): 0.001433
  • LIPO(Sec/SPII): 0.010221
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 151220244 NCBI
• RefSeq: YP_001331067 NCBI
• UniProt: A0A0H3K614 UniProt

⊟Protein sequence

• MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQLLGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETYLVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGILYKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNMMIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAILSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMNTVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLLVNKTDKIKYAHKEE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications