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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
  • pan locus tag?: SAUPAN000385000
  • symbol: NWMN_0032
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
  • symbol: NWMN_0032
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 44597..45817
  • length: 1221
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACTGATTTGTTGAAAATTAAAAACTTCAGATTGTTCTTTTTAGCAGAAATAATTTCA
    GCATTCGGAGTTGGAATTAGTACAGTTGGGGCTAATTGGTATTTAATCGACAAAACTAAC
    GACTCCCAACTATTAGGTATTATGTTAGCTTTAAATGTATTATCCGGTTTCTTAGCTTCT
    CCTATAATAGGTGGACTAGCCGATAAATATAATAGACGAAATATTATTTTAATCACCTAT
    CTTCTTCAGGTAATATTATATTTACTCATTGTCATAGCACTCGTAATGATAGGATTTGAG
    ACCTATTTAGTGATTGGATTTGCGATTGTTAATGGGATAGGTTGGACTACGTATATGGCA
    ACATCTAGAAGTTTAGTTAAACAGATATTAAAACCAGATCAATATACAGATGCAAATTCT
    CTATTAGAGATTAGTTTGCAAACAGGTATGTTTATTGCAGGTGGATTATCAGGAATATTA
    TATAAAATAAATGGATTCACTCTAATTATAGCGATGACTATAATGATGTTTCTAATCAGC
    ATTTTTATGTTATTTAGATTGCATGTAGATAAACCAACTCATTCAGAGGAAGAATCAACA
    AATAGCTTATTGCAAGAATATTTATTAGGATGGAAATTTTTAAAAGACAACATGATGATA
    TTTATTTTTGGAGTTATTTCAATCATACCAATGGTGTTTACAATGATCTTTAACATATCA
    TTACCAGGATATGTTTATAATGTTTTAAAACTTTCGTCTGTACAATTTGGTTTTTCAGAT
    ATGTTATATGGCATTGGAGGATTATGTGCAGGTTTAATTTCAGCTATTCTTTCGAAGAAA
    ATCTCAACTAAAGTATTGATATTTTTGTTATATTTCATATTAGTCATAAATTCAGCACTA
    TTTATTTGGATAAACTCAGCATTTTACTTATTCATAGGATCATTTATACTAGGCTACTCA
    ATTTCATCAATCAGAATTTATATGAATACAGCTATAATGAACACTGTTTCAGATAAATAT
    GTCGGTCGCTCATTTACGATATGGACGTCAATTTCATTGTTACTGCAAAGTTTAATTGCT
    CCATTTTTAGGAAGATGGATTAATGAAATTAATGATAAATTCGGTTTCTATATTATACTC
    ATTTTATCCTTACTCATATTTGTTACACTGCTGCTTGTTAACAAAACAGACAAAATAAAA
    TATGCACATAAAGAAGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1221

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0032 [new locus tag: NWMN_RS00205 ]
  • symbol: NWMN_0032
  • description: hypothetical protein
  • length: 406
  • theoretical pI: 9.43757
  • theoretical MW: 45716.5
  • GRAVY: 0.86798

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 165.1)
    and 8 more
    multidrug resistance protein (TIGR00880; HMM-score: 67.8)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 26.6)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 16.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 12.6)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 8.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar (Glycoside-Pentoside-Hexuronide) transporter (TIGR00792; HMM-score: 7.9)
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 109)
    and 4 more
    MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 60)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 30.5)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.7)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 9.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024105
    • TAT(Tat/SPI): 0.001433
    • LIPO(Sec/SPII): 0.010221
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQLLGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETYLVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGILYKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNMMIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAILSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMNTVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLLVNKTDKIKYAHKEE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]