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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0047 [new locus tag: NWMN_RS00275 ]
  • pan locus tag?: SAUPAN000893000
  • symbol: NWMN_0047
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0047 [new locus tag: NWMN_RS00275 ]
  • symbol: NWMN_0047
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 61194..62381
  • length: 1188
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGGATATGAATCAACAATTAATTGAAACTTTAAAATCTAAAGAAGGCAAAATGATT
    GAGATCAGACGTTATTTACATCAGCATCCAGAATTATCTTTTCATGAAGATGAAACGGCG
    AAATACATCGCTGAATTTTACAAAGGTAAAGATGTGGAAGTAGAAACGAATGTCGGACCA
    CGTGGAATTAAAGTAACGATTGATTCAGGGAAACCTGGTAAAACATTAGCAATCCGTGCA
    GACTTTGACGCATTACCCATTACTGAAGATACAGGATTATCTTTTGCATCACAAAATAAA
    GGTGTTATGCACGCATGTGGTCACGATGCACATACAGCATACATGCTTGTATTAGCAGAG
    ACGCTTGCTGAAATGAAAGATAGTTTTACAGGAAAAGTCGTTGTGATACATCAACCAGCT
    GAAGAAGTACCACCAGGTGGTGCTAAAACAATGATTGAAAATGGTGTATTAGACGGTGTT
    GATCATGTATTAGGTGTACACGTCATGAGCACAATGAAAACAGGTAAAGTGTATTACAGA
    CCTGGTTATGTTCAAACAGGACGCGCATTCTTCAAATTGAAAGTTCAAGGTAAAGGTGGT
    CATGGTTCATCACCACATATGGCCAATGATGCCATTGTTGCAGGTAGCTACTTCGTCACA
    GCGTTACAAACAGTTGTATCTAGACGACTAAGTCCATTTGAAACCGGTGTTGTCACAATC
    GGTTCATTTGACGGTAAAGGTCAATTCAATGTCATTAAAGATGTTGTTGAAATTGAAGGT
    GATGTACGTGGATTAACAGATGCTACAAAAGCAACAATTGAAAAAGAAATTAAACGTTTA
    TCAAAAGGATTAGAGGATATGTATGGTGTAACTTGCACCTTAGAATATAACGATGATTAT
    CCAGCATTATATAATGATCCAGAGTTTACTGAGTACGTGGCTAAGACGTTGAAAGAAGCA
    AACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATAT
    TATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAA
    ATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCA
    GAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0047 [new locus tag: NWMN_RS00275 ]
  • symbol: NWMN_0047
  • description: hypothetical protein
  • length: 395
  • theoretical pI: 5.44053
  • theoretical MW: 43472
  • GRAVY: -0.322785

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 349.9)
    and 5 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 44.2)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 32.7)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 28.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 24.5)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 14.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 109.2)
    and 1 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 36.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003325
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000473
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDMNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFYKGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHACGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHVLGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTALQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKRLSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSEDFAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1] [2]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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