PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0076 [new locus tag: NWMN_RS00430 ]
• pan locus tag^?: SAUPAN000941000
• symbol: NWMN_0076
• pan gene symbol^?: sscE
• synonym:
• product: polysaccharide extrusion protein

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0076 [new locus tag: NWMN_RS00430 ]
• symbol: NWMN_0076
• product: polysaccharide extrusion protein
• replicon: chromosome
• strand: +
• coordinates: 97765..99195
• length: 1431
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329966 NCBI
• RefSeq: YP_001331111 NCBI
• BioCyc:
• MicrobesOnline: 3705607 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
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  481
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  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGAAGAGTGATTCACTAAAAGAAAATATTATTTATCAAGGGCTATACCAATTGATTAGA
  ACGATGACACCACTGATTACAATACCCATTATTTCACGTGCATTTGGTCCCAGTGGTGTG
  GGTATTGTTTCATTTTCTTTCAATATCGTGCAATACTTTTTGATGATTGCAAGTGTTGGC
  GTTCAGTTATATTTTAATAGAGTTATCGCGAAGTCCGTTAACGACAAACGGCAATTGTCA
  CAGCAGTTTTGGGATATCTTTGTCAGTAAATTATTTTTAGCGTTAACAGTTTTTGCGATG
  TATATGGTCGTAATTACTATATTTATTGATGATTACTATCTTATTTTCCTACTACAAGGA
  ATCTATATTATAGGTGCAGCACTCGATATTTCATGGTTTTATGCTGGAACTGAAAAGTTT
  AAAATTCCTAGCCTCAGTAATATTGTTGCGTCTGGTATTGTATTAAGTGTAGTTGTTATT
  TTTGTCAAAGATCAATCAGATTTATCATTGTATGTATTTACTATTGCTATTGTGACGGTA
  TTAAACCAATTACCTTTGTTTATCTATTTAAAACGATACATTAGCTTTGTTTCGGTTAAT
  TGGATACACGTCTGGCAATTGTTTCGTTCGTCATTAGCATACTTATTACCAAATGGACAG
  CTCAACTTATATACTAGTATTTCTTGCGTTGTTCTTGGTTTAGTAGGTACATACCAACAA
  GTTGGTATCTTTTCTAACGCATTTAATATTTTAACGGTCGCAATCATAATGATTAATACA
  TTTGATCTTGTAATGATTCCGCGTATTACCAAAATGTCTATCCAGCAATCACATAGTTTA
  ACTAAAACGTTAGCTAATAATATGAATATTCAATTGATATTAACAATACCTATGGTCTTT
  GGTTTAATTGCAATTATGCCATCATTTTATTTATGGTTCTTTGGTGAGGAATTCGCATCA
  ACTGTCCCATTGATGACCATTTTAGCGATACTTGTATTAATCATTCCTTTAAATATGTTG
  ATAAGCAGGCAATATTTATTAATAGTGAATAAAATAAGATTATATAATGCGTCAATTACT
  ATTGGTGCAGTGATAAACCTAGTATTATGTATTATTTTGATATATTTTTATGGAATTTAC
  GGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGAT
  ATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCT
  GTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCT
  ACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAA
  AATCAATACGTATGGCAAATATTGAGGCATCTTCGACATAAAACAATTTAA
  60
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  1431
  

⊟Protein

⊟General

• locus tag: NWMN_0076 [new locus tag: NWMN_RS00430 ]
• symbol: NWMN_0076
• description: polysaccharide extrusion protein
• length: 476
• theoretical pI: 9.82285
• theoretical MW: 54161.1
• GRAVY: 1.00546

⊟Function

• TIGRFAM:
• TheSEED  :
  • Membrane protein involved in the export of O-antigen, teichoic acid lipoteichoic acids
  Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Membrane protein involved in the export of O-antigen, teichoic acid lipoteichoic acids
• PFAM:
  MviN_MATE (CL0222) Polysacc_synt; Polysaccharide biosynthesis protein (PF01943; HMM-score: 70.1)
  and 1 more
  Polysacc_synt_3; Polysaccharide biosynthesis protein (PF13440; HMM-score: 18.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 13
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9998
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0002
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024133
  • TAT(Tat/SPI): 0.001017
  • LIPO(Sec/SPII): 0.004909
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 151220288 NCBI
• RefSeq: YP_001331111 NCBI
• UniProt: A0A0H3KA03 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYMVVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVIFVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQSHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFFLLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0074 > NWMN_0075 > NWMN_0076

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications