From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0085 [new locus tag: NWMN_RS00480 ]
  • pan locus tag?: SAUPAN000955000
  • symbol: NWMN_0085
  • pan gene symbol?: phnE2
  • synonym:
  • product: phosphonates ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0085 [new locus tag: NWMN_RS00480 ]
  • symbol: NWMN_0085
  • product: phosphonates ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 107296..108096
  • length: 801
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCTTTAGAAATACCTACAAAGTATGACTCCCTTTTAAAGAAAAAGGTTTCTTTAAAA
    ACGAGTTTTACCTTCATGTTAATCATTGTGCTTATCATTTGGAGCTTCATTTATACGGGA
    TTTAATTTTGGAGATTTAATGATAGGAATACCTCAAATAGGTGATCTATTCAAACAAATG
    ATTCCACCTGATTTCGAGTATTTACAACAAATTACAACGCCAATGTTAGATACCATTCGA
    ATGGCTATCGTAAGTACAGTATTAGGTAGCATCGTTTCAATACCAATTGCGTTATTATGT
    GCTAGCAATATCGTTCATCAAAAGTGGATTTCAATACCCTCGCGCTTTATTTTAAATATA
    GTTCGTACTATTCCAGATTTGTTATTAGCAGCAATCTTTGTGGCTGTATTTGGAATCGGT
    CAAATTCCAGGGATATTAGCACTGTTTATTTTAACTATCTGTATTATTGGAAAATTATTA
    TATGAATCATTGGAAACGATAGATCCAGGTCCAATGGAAGCAATGACGGCTGTTGGCGCT
    AATAAAATAAAATGGATTGTTTTCGGTGTTGTACCACAAGCCATATCGTCATTTATGTCA
    TACGTATTATATGCATTTGAAGTAAATATACGTGCTTCAGCTGTGCTTGGATTAGTCGGC
    GCTGGCGGTATTGGATTGTTTTATGATCAAACACTTGGTTTATTTCAATATCCAAAAACA
    GCAACGATTATTTTATTTACTTTAGTTATCGTCGTCGTCATTGATTACATCAGTACGAAA
    GTGAGGGCACATCTCGCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0085 [new locus tag: NWMN_RS00480 ]
  • symbol: NWMN_0085
  • description: phosphonates ABC transporter permease
  • length: 266
  • theoretical pI: 8.61947
  • theoretical MW: 29281.1
  • GRAVY: 0.957519

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 307.8)
    and 4 more
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 42.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 23.6)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 18.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 18.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 54.9)
    and 1 more
    Acyl_transf_3 (CL0316) DUF418; Protein of unknown function (DUF418) (PF04235; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004544
    • TAT(Tat/SPI): 0.00022
    • LIPO(Sec/SPII): 0.001419
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFGDLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCASNIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLLYESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGLVGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0085&oldid=53919"