PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0200 [new locus tag: NWMN_RS01115 ]
• pan locus tag^?: SAUPAN001146000
• symbol: bglA
• pan gene symbol^?: bglA
• synonym:
• product: 6-phospho-beta-glucosidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0200 [new locus tag: NWMN_RS01115 ]
• symbol: bglA
• product: 6-phospho-beta-glucosidase
• replicon: chromosome
• strand: +
• coordinates: 257003..258439
• length: 1437
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330049 NCBI
• RefSeq: YP_001333691 NCBI
• BioCyc:
• MicrobesOnline: 3705731 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACAAAATTACCGCAAAATTTCATGTGGGGTGGCGCTCTTGCCGCAAATCAATTTGAA
  GGTGGATATGATAAAGGTGGTAAAGGGTTAAGTGTAATTGATGTTATGACGAGTGGTGCA
  CATGGCAAAGCACGTCAGATTACAGAATCTATAGATCCCAATCACTATTATCCAAATCAT
  GAAGGTATTGATTTTTATCATCGTTATAAGGAAGATATTGCCTTGTTTAAAGAAATGGGA
  TTGAAATGTTTACGTACGTCGATTGCGTGGACACGTATCTTTCCGAATGGGGATGAAGAT
  GTGCCAAATGAAGAAGGACTCGCCTTTTATGATCGTATCTTTGATGAATTAATTGCACAA
  GGTATTGAACCTGTTGTGACGTTATCACATTTTGAGATGCCACTTCATTTAGCGAAACAT
  TATGGTGGATTTAGAAATAGAGAAGTTGTCGATTATTTTGTGCATTTTGCGCGTGTTGTA
  TTTGAAAGATATAAAGATAAAGTTACATATTGGATGACGTTTAATGAAATTAATAATCAG
  ATGGACACATCAAATCCTATCTTTTTATGGACGAATTCTGGGGTAGCATTGACAGAAAAT
  GATAATCCTGAAGAAGTCTTGTATCAAGTAGCACATCATGAACTTTTAGCCAGTGCTTTA
  GCAGTTCGTCTTGGTAAAGAGATTAATCCGAAGTTTAAGATTGGAACAATGATTTCACAT
  GTACCCATTTATCCATATTCGTGTCATCCGAAAGATATGATGGAAGCACAAATTGCGAAT
  CGCTTACGTTTCTTTTTCCCGGATGTCCAAGTGAGAGGTTATTATCCAAGCTATGCTAAA
  AAAATGTTGGCACGAAAAGGATATGATGTTGGATGGCAAGAAGGGGACGACAGTATTTTA
  CAGCAGGGCACGGTTGATTATATTGGCTTTAGTTATTACATGTCTACGGCTGTAAAACAT
  GATGTTGATACTACAGTTGAAAACAACATCGTCAACGGTGGTTTGAATCATTCTGTGGAG
  AATCCGCATATCGCAACGAGTGATTGGGGTTGGGCGATTGATCCAGATGGCTTAAGATAT
  ACATTGAATGTGTTATATGATCGTTATCAGTTACCACTTTTTATTGTGGAAAATGGTTTT
  GGTGCAGTTGATGAAGTGGTAGATGGACATATTCATGATGATTATCGCATTGAATATTTA
  AAAGCACATATTACAGCAGCGATAGAAGCAGTTGATCAAGATGGTGTAGATTTAATCGGT
  TATACACCGTGGGGAATCATTGATATTGTTTCATTTACAACCGGTGAAATGAAGAAACGC
  TATGGTTTAATATATGTTGATCGAGATAATGATGGTCATGGCACGATGGAACGCTTGAAA
  AAAGATTCGTTCTATTGGTATCAACAAGTGATAGCATCAAATGGAGATAAATTATAA
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⊟Protein

⊟General

• locus tag: NWMN_0200 [new locus tag: NWMN_RS01115 ]
• symbol: BglA
• description: 6-phospho-beta-glucosidase
• length: 478
• theoretical pI: 5.11616
• theoretical MW: 54708.2
• GRAVY: -0.373222

⊟Function

• TIGRFAM:
  beta-galactosidase (TIGR03356; HMM-score: 306.6)
  and 1 more
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 6-phospho-beta-galactosidase (TIGR01233; EC 3.2.1.85; HMM-score: 215.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Glyco_hydro_tim (CL0058) Glyco_hydro_1; Glycosyl hydrolase family 1 (PF00232; HMM-score: 445.1)
  and 1 more
  Cellulase; Cellulase (glycosyl hydrolase family 5) (PF00150; HMM-score: 16.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.111339
  • TAT(Tat/SPI): 0.006057
  • LIPO(Sec/SPII): 0.021288
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220412 NCBI
• RefSeq: YP_001333691 NCBI
• UniProt: A0A0H3KAA4 UniProt

⊟Protein sequence

• MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKARQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVPNEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVVFERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLASALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYPSYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGGLNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIHDDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDNDGHGTMERLKKDSFYWYQQVIASNGDKL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0199 > bglA

⊟Regulation

• regulator: BglR* (repression) regulon

  BglR*

  (TF)

  important in Beta-glucosides utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications