COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
• pan locus tag^?:
• symbol: int
• pan gene symbol^?: —
• synonym:
• product: integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
• symbol: int
• product: integrase
• replicon: chromosome
• strand: -
• coordinates: 320846..322051
• length: 1206
• essential: unknown

⊟Accession numbers

• Gene ID: 5331946 NCBI
• RefSeq: YP_001331297 NCBI
• BioCyc:
• MicrobesOnline: 3705794 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGTGGTTTGAAAAATTTAAAAATAAGAACAATGAAACGAAGTATAGATACTACGAGAAA
  TACAAAGATCCGTATACAGATAAATGGAAACGTGTAAGTGTTGTCTTGAATAAGAATACA
  AAGCAATCGCAAAAAGAGGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
  AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGTTATTAGATGAATGGCTT
  GAATATCATATAAAAACATCTGGCTTTAAAGTAACGACGCTTGATAATTTGAAAACAAGA
  ATCAAAAACATCAAAAAGAACAGTTCTCAAAATTTACTTTTAAACAAAATTGATACAAAG
  TACATGCAAACATTTATTAACGAATTATCAAACGTATATTCTGCAAATCAGGTAAAGCGT
  CAACTTGGACATATGAAAGAAGCTATTAAATACGCCGTTAAATTTTACAATTATCCAAAC
  GAACACATATTAAATAGCGTCACACTACCAAAGAAGAGTAAGACGATAGAAGATATAGAA
  AAAGAAGAAGCGAAAATGTACAACTATTTAGAGATGGAACAGGTAATACAGATACGCGAT
  TTTATACTGAACGATAATAACATGCAGTATAGAGCTCGTATTTTAGTTGCTGGGGCTGTA
  GAAGTTCAAGCTTTAACAGGTATGCGCATAGGTGAGTTATTAGCTCTCCAAGTTAAAGAT
  GTTGACCTCAAAAATAAAACGATCGCTATTAATGGCACTATTCACAGAATCAAATGTAAT
  GCTGGATTTGGTCACAAAGATACTACGAAGACCGCAGGTTCAAAAAGAAAAATCGCCATC
  AATTCAAGGATAGCAAATGTATTGAAAAAAATAATGTTAGAAAATAAAAAGATGCAACAA
  TGGGAACCAAGCTATGTTGATAGAGGGTTTATATTCACAACTTGCCAAGGAAATCCTATG
  CAAGGCAGTAGGATAAACAAACGATTGTCCTCAGCTGCAGAATCATTAAATATAAATAAA
  AAAGTTACTACTCACACACTAAGGCATACACACATAAGTTTATTGGCGGAAATGAATATA
  TCGTTAAAAGCAATTATGAAAAGAGTAGGACATAGAGATGAAAAAACGACTATAAAGGTG
  TATACACATGTAACAGAGAAAATGGACAGAGAGTTAGAGCAAAAATTAGAAAAACTTGTG
  TACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1206
  

⊟Protein

⊟General

• locus tag: NWMN_0263 [new locus tag: NWMN_RS01450 ]
• symbol: Int
• description: integrase
• length: 401
• theoretical pI: 10.3973
• theoretical MW: 46984.2
• GRAVY: -0.694015

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 74)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 70.6)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 54.6)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 54.6)
• TheSEED:
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 100.1)
  and 4 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 23.5)
  HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 16.2)
  l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 14.5)
  GHD (CL0369) DUF3372; Domain of unknown function (DUF3372) (PF11852; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01108
  • TAT(Tat/SPI): 0.000421
  • LIPO(Sec/SPII): 0.003012
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220475 NCBI
• RefSeq: YP_001331297 NCBI
• UniProt: A0A0H3KAG8 UniProt

⊟Protein sequence

• MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGFKVTTLDNLKTRIKNIKKNSSQNLLLNKIDTKYMQTFINELSNVYSANQVKRQLGHMKEAIKYAVKFYNYPNEHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAGAVEVQALTGMRIGELLALQVKDVDLKNKTIAINGTIHRIKCNAGFGHKDTTKTAGSKRKIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAESLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHRDEKTTIKVYTHVTEKMDRELEQKLEKLVY

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications