PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0271 [new locus tag: NWMN_RS01520 ]
• pan locus tag^?: SAUPAN000547000
• symbol: NWMN_0271
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0271 [new locus tag: NWMN_RS01520 ]
• symbol: NWMN_0271
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 327325..328113
• length: 789
• essential: unknown

⊟Accession numbers

• Gene ID: 5330110 NCBI
• RefSeq: YP_001331305 NCBI
• BioCyc:
• MicrobesOnline: 3705802 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACTGAGGAAAAACAAGAACCACAAGAAAAAGTAAGCATACTCAAAAAACTAAAGATA
  AATAATATCGCTGAGAAAAATAAAAGGAAATTCTATAAATTTGCAGTATACGGAAAAATT
  GGCTCAGGAAAAACCACGTTTGCTACAAGAGATAAAGACGCTTTCGTCATTGACATTAAC
  GAAGGTGGAACAACGGTTACTGACGAAGGATCAGACGTAGAAATCGAGAACTATCAACAC
  TTTGTTTATGTTGTAAATTTTTTACCTCAAATTTTACAGGAGATGAGAGAAAACGGACAA
  GAAATCAATGTTGTAGTTATTGAAACTATTCAAAAACTTAGAGATATGACATTGAATGAT
  GTGATGAAAAATAAGTCTAAAAAACCAACGTTTAATGATTGGGGAGAAGTTGCTGAACGA
  ATTGTCAGTATGTACAGATTAATAGGAAAACTTCAAGAAGAATACAAATTCCACTTTGTT
  ATTACAGGTCATGAAGGTATCAACAAAGATAAAGATGATGAAGGTAGCACTATCAACCCT
  ACTATCACTATTGAAGCGCAAGAACAAATTAAAAAAGCTATTACTTCTCAAAGTGATGTG
  TTAGCTAGGGCAATGATTGAAGAATTTGATGATAACGGAGAAAAGAAAGCTAGATATATT
  CTAAACGCTGAACCTTCTAATACGTTTGAAACAAAGATTAGACATTCACCTTCAATAACA
  ATTAACAATAAGAAATTTGCAAATCCTAGCATTACGGACGTAGTAGAAGCAATTAGAAAT
  GGAAACTAA
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⊟Protein

⊟General

• locus tag: NWMN_0271 [new locus tag: NWMN_RS01520 ]
• symbol: NWMN_0271
• description: hypothetical protein
• length: 262
• theoretical pI: 5.81837
• theoretical MW: 29956.7
• GRAVY: -0.657252

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage nucleotide-binding protein (TIGR01618; HMM-score: 26.5)
• TheSEED:
• PFAM:
  P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 111.6)
  and 5 more
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 19.6)
  AAA_22; AAA domain (PF13401; HMM-score: 14.6)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 13.5)
  no clan defined Gasdermin; Gasdermin family (PF04598; HMM-score: 11.8)
  P-loop_NTPase (CL0023) AAA_17; AAA domain (PF13207; HMM-score: 8.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01491
  • TAT(Tat/SPI): 0.008703
  • LIPO(Sec/SPII): 0.000958
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220483 NCBI
• RefSeq: YP_001331305 NCBI
• UniProt: A0A0H3K9E4 UniProt

⊟Protein sequence

• MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGKTTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEINVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFVITGHEGINKDKDDEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKARYILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0271 > NWMN_0272 > NWMN_0273

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications