PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0285 [new locus tag: NWMN_RS01630 ]
• pan locus tag^?: SAUPAN000557000
• symbol: NWMN_0285
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0285 [new locus tag: NWMN_RS01630 ]
• symbol: NWMN_0285
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 336545..336781
• length: 237
• essential: unknown

⊟Accession numbers

• Gene ID: 5330124 NCBI
• RefSeq: YP_001331319 NCBI
• BioCyc:
• MicrobesOnline: 3705816 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  
  ATGATTAACATACCTAAAATGAAATTCCCGAAAAAGTACACTGAAATAATCAAAAAATAT
  AAAAATAAAACACCTGAAGAAAAAGCTAAGATTGAAGATGATTTCATTAAAGAAATTAAT
  GATAAAGACAGTGAATTTTACAGTCCTATGATGGCTAATATGAATGAACATGAATTAAGG
  GCTATGTTAAGAATGATGCCTAGTTTAATTGATACTGGAGATGGCAATGATGATTAA
  60
  120
  180
  237
  

⊟Protein

⊟General

• locus tag: NWMN_0285 [new locus tag: NWMN_RS01630 ]
• symbol: NWMN_0285
• description: hypothetical protein
• length: 78
• theoretical pI: 5.17686
• theoretical MW: 9246.67
• GRAVY: -0.984615

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage protein
• PFAM:
  no clan defined DUF7366; Domain of unknown function (DUF7366) (PF24074; HMM-score: 170)
  and 4 more
  EsxAB (CL0352) LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 14)
  no clan defined DUF5601; Domain of unknown function (DUF5601) (PF18151; HMM-score: 13.2)
  Curto_V2; Curtovirus V2 protein (PF07325; HMM-score: 12.4)
  5_3_exonuc_C (CL0464) XPG_I_2; XPG domain containing (PF12813; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8588
  • Cytoplasmic Membrane Score: 0.005
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.1359
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004101
  • TAT(Tat/SPI): 0.000323
  • LIPO(Sec/SPII): 0.000569
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220497 NCBI
• RefSeq: YP_001331319 NCBI
• UniProt: A0A0H3KE53 UniProt

⊟Protein sequence

• MINIPKMKFPKKYTEIIKKYKNKTPEEKAKIEDDFIKEINDKDSEFYSPMMANMNEHELRAMLRMMPSLIDTGDGNDD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0285 > NWMN_0286

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications