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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman
locus tag: NWMN_0330 [new locus tag: NWMN_RS01875 ]
pan locus tag^?: SAUPAN001874000
symbol: NWMN_0330
pan gene symbol^?: —
synonym:
product: glyoxalase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_0330 [new locus tag: NWMN_RS01875 ]
symbol: NWMN_0330
product: glyoxalase family protein
replicon: chromosome
strand: +
coordinates: 380696..381622
length: 927
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 5330169 NCBI
RefSeq: YP_001331364 NCBI
BioCyc:
MicrobesOnline: 3705861 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGAATATAGTAGGGCATCATCACATATCCATGTATACAAAAGATGCAAAACGTAATAAG
GATTTTTACACAAATGTCCTTGGATTACGATTAGTTGAAAAGTCGGTTAATCAAGACAAT
CCTTCAATGTATCATTTGTTTTATGGGGACGAAGTAGGTACAGCCGGAACAATTTTAAGC
TTTTTTGAAATTCCCAATGCGGGTCATAAGCAGCCAGGTACTGAAACGATTTATCGATTT
TCATTATTAGTACCAAATCAAGCGGCACTTCATTATTTTGAAAAACGTCTTGAGAATAAT
GGTATTAAGTCTGAACGTTTGTACTATCTTGGACAAGAAGGTGTTGTCTTTAAAGATGAA
GACGACTTAGAAATCATATTGCTTGTTAATGATAGTTTTGAAGTACCACATCAATGGCAA
CATAACGCTTATAGTGAAATACCTCAAGCATATCAAATTTTAGGAATAGGGCCAGTCGAA
TTAAGAGTTAGAAATGCAGCGCGTACGGTAGAATTTTTGGAAAATGTCTTAGGTTATCGC
AAAAGAGATAATAAATCATTCGATGTGCTGACATTAGCACCACAAGGTTTATATTCGGAT
TTTGTAGTTATTGAGCAACAGGGACAACGTGAAAGACCTGGACGAGGTTATATCCATCAT
ATTGCAGTTAATACACCACAAATGAGTGACTTAGATGCAATTTACAAGAAATTACAACAA
CAACCACAAAGTAATTCAGGTATAATTGATCGCTATTTCTTTAAATCATTATACTATCGC
CATAATTCAATTATGTATGAATTTGCGACTGAAGCGCCTGGATTTACTATTGATACACCT
GTTGAACAATTAGGAAGTCAATTGAACTTGCCTGACTTTTTAGAAGCAGAACGTGAACAA
ATTGAAAGTAAGTTACACGAAATATAA
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927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_0330 [new locus tag: NWMN_RS01875 ]
symbol: NWMN_0330
description: glyoxalase family protein
length: 308
theoretical pI: 5.74304
theoretical MW: 35578.8
GRAVY: -0.494805

⊟Function[edit | edit source]

TIGRFAM:
Metabolism Energy metabolism Other catechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 28.6)
Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 25.4)
Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 25.4)
and 3 more
3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 20.4)
Metabolism Energy metabolism Other 2,3-dihydroxybiphenyl 1,2-dioxygenase (TIGR03213; EC 1.13.11.39; HMM-score: 19.5)
methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 15.6)
TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
PFAM:
Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 73.7)
and 2 more
Glyoxalase_4; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF13669; HMM-score: 19.1)
CppA_N; CppA N-terminal (PF14506; HMM-score: 17.6)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004623
- TAT(Tat/SPI): 0.000202
- LIPO(Sec/SPII): 0.000616
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 151220542 NCBI
RefSeq: YP_001331364 NCBI
UniProt: A0A0H3KE96 UniProt

⊟Protein sequence[edit | edit source]

MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMYHLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGIKSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVELRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYIHHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFTIDTPVEQLGSQLNLPDFLEAEREQIESKLHEI

⊟Experimental data[edit | edit source]

experimentally validated:
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: NWMN_0330 > NWMN_0331 > NWMN_0332

⊟Regulation[edit | edit source]

regulator: SigB (activation) regulon
SigB (sigma factor) controlling a large regulon involved in stress/starvation response and adaptation; ^[1] other strains

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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