PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
• pan locus tag^?: SAUPAN002215000
• symbol: tmk
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
• symbol: tmk
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 508675..510012
• length: 1338
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331632 NCBI
• RefSeq: YP_001331479 NCBI
• BioCyc:
• MicrobesOnline: 3705979 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
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  1021
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  1141
  1201
  1261
  1321
  
  ATGAAGCAACCTATTTTAAATAAATTAGAAAGTTTAAATCAAGAAGAAGCGATTTCTTTG
  CATGTTCCGGGTCATAAAAATATGACTATCGGTCATTTATCTCAATTATCAATGACAATG
  GATAAAACTGAAATACCTGGATTAGATGATTTACATCATCCTGAAGAAGTCATTTTGGAA
  AGTATGAAGCAGGTGGAGAAACATTCAGATTATGATGCTTATTTCTTAGTGAATGGCACC
  ACTTCAGGAATATTATCTGTCATCCAGTCTTTTTCACAGAAAAAAGGCGATATCTTAATG
  GCAAGAAATGTACATAAATCTGTGTTACATGCGCTCGATATTAGCCAACAAGAAGGGCAT
  TTTATTGAAACGCATCAAAGTCCGTTAACGAATCATTATAATAAAGTTAATTTAAGCCGT
  TTGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACA
  TTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGAC
  GAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTCTACATTAAATTATCAA
  GCTGACTATGTTGTTCAATCTTTTCATAAAACGTTACCAGCTTTAACGATGGGCTCGGTA
  CTTTATATTCATAAAAATGCACCTTATAGAGAAACTATTATAGAATATCTAAGCTACTTC
  CAAACATCTAGTCCTTCGTATTTGATTATGGCTAGTTTAGAGTCAGCTGCCGAGTTCTAT
  AAAACATATGATAGTACCGTGTTTTTTGATAAGAGAGCGCAATTAATCGAATGTTTGGAG
  AAGAAGGGTTTTGAAATGCTTCAAGTTGATGATCCGTTGAAGTTGCTGATAAAATATGAA
  GGTTTTACAGGTCATGATATTCAAAATTGGTTTATGAATGCACATATCTATTTAGAATTA
  GCGGACGACTATCAAGCATTAGCGATATTGCCGTTATGGCATCATGATGATACGTATTTA
  TTTGATTCGCTTTTACGTAAAATTGAAGATATGATTTTACCGAAAAAATCAGTTTCTAAA
  GTTAAACAAACACAACTTTTAACAACTGAAGGTAACTATAAACCAAAACGCTTTGAATAT
  GTTACTTGGTGTGATTTGAAAAAGGCAAAAGGTAAAGTTCTGGCGCGACATATTGTCCCG
  TATCCGCCAGGGATTCCTATTATTTTCAAAGGAGAAACAATAACTGAAAATATGATAGAA
  TTGGTAAATGAATATCTGGAAACTGGAATGATAGTTGAAGGAATTAAAAATAATAAAATT
  TTAGTTGAGGATGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0445 [new locus tag: NWMN_RS02535 ]
• symbol: Tmk
• description: hypothetical protein
• length: 445
• theoretical pI: 6.02953
• theoretical MW: 51021
• GRAVY: -0.29618

⊟Function

• TIGRFAM:
  ornithine decarboxylase SpeF (TIGR04301; EC 4.1.1.17; HMM-score: 29.9)
  O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 29)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 26.5)
  putative ornithine decarboxylase (TIGR04318; EC 4.1.1.-; HMM-score: 25.9)
  Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 25.1)
  and 2 more
  Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 15.3)
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 11.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLP_aminotran (CL0061) OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 146.2)
  and 3 more
  no clan defined OKR_DC_1_C; Orn/Lys/Arg decarboxylase, C-terminal domain (PF03711; HMM-score: 35)
  PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 26.4)
  Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 18.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003136
  • TAT(Tat/SPI): 0.000237
  • LIPO(Sec/SPII): 0.000308
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220657 NCBI
• RefSeq: YP_001331479 NCBI
• UniProt: A0A0H3K732 UniProt

⊟Protein sequence

• MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTEIPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMARNVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGETFNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMGSVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLIECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWHHDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGKVLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: tmk > tmk > NWMN_0447

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications