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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
  • pan locus tag?: SAUPAN002289000
  • symbol: NWMN_0486
  • pan gene symbol?: mcsB
  • synonym:
  • product: ATP:guanido phosphotransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
  • symbol: NWMN_0486
  • product: ATP:guanido phosphotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 558119..559129
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATGACGCATAATATTCATGATAATATCAGCCAATGGATGAAAAGTAATGAAGAAACA
    CCAATTGTTATGTCTTCTAGAATTCGGTTAGCGCGTAATTTAGAAAATCATGTGCATCCA
    CTAATGTATGCTACTGAAAATGATGGATTTAGAGTTATAAATGAGGTACAAGATGCCTTG
    CCAAACTTTGAATTAATGCGTCTTGATCAAATGGATCAACAAAGTAAAATGAAAATGGTT
    GCAAAGCATTTGATTAGTCCTGAACTAATAAAACAACCAGCAGCCGCAGTATTAGTGAAT
    GACGATGAATCTTTAAGTGTCATGATAAATGAAGAGGACCATATTCGTATTCAAGCTATG
    GGAACTGACACGACATTACAGGCTTTATATAATCAAGCTTCATCAATTGATGATGAATTA
    GATCGAAGCCTTGATATAAGTTATGATGAACAACTTGGTTATTTAACTACATGTCCTACC
    AATATAGGTACTGGTATGAGAGCAAGCGTGATGCTACATTTGCCAGGTCTATCTATTATG
    AAAAGAATGACACGGATTGCTCAAACCATTAATCGTTTTGGATATACAATCAGAGGTATT
    TACGGTGAAGGTTCGCAAGTTTATGGACATACTTATCAAGTATCCAACCAACTTACACTT
    GGTAAATCTGAGTTAGAAATCATAGAAACATTAACAGAAGTTGTTAATCAAATCATTCAT
    GAAGAAAAACAAATACGACAAAAGTTAGACACTTATAATCAATTAGAAACACAAGACCGT
    GTTTTTCGCTCGCTAGGTATTTTACAAAACTGTAGAATGATAACTATGGAAGAGGCTTCT
    TATAGATTAAGCGAAGTTAAACTTGGTATAGATTTAAATTACATTGAATTACAAAACTTT
    AAATTTAATGAATTGATGGTAGCTATACAGTCACCATTTTTATTAGATGAAGAAGATGAC
    AAATCTGTAAAAGAAAAACGAGCAGATATACTAAGAGAACATATAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
  • symbol: NWMN_0486
  • description: ATP:guanido phosphotransferase
  • length: 336
  • theoretical pI: 4.87485
  • theoretical MW: 38741
  • GRAVY: -0.410714

Function[edit | edit source]

  • reaction:
    EC 2.7.14.1?  ExPASy
    Protein arginine kinase ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine
  • TIGRFAM:
  • TheSEED:  
    Protein-arginine kinase McsB (EC 2.7.14.1)
  • PFAM:
    GCS (CL0286) ATP-gua_Ptrans; ATP:guanido phosphotransferase, C-terminal catalytic domain (PF00217; HMM-score: 224.2)
    and 1 more
    no clan defined Pas_Saposin; Pas factor saposin fold (PF09016; HMM-score: 11.8)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002442
    • TAT(Tat/SPI): 0.000181
    • LIPO(Sec/SPII): 0.000286
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MMTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYATENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDESLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTNIGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLTLGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITMEEASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHIK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Darren J Wozniak, Kiran B Tiwari, Rami Soufan, Radheshyam K Jayaswal
The mcsB gene of the clpC operon is required for stress tolerance and virulence in Staphylococcus aureus.
Microbiology (Reading): 2012, 158(Pt 10);2568-2576
[PubMed:22902728] [WorldCat.org] [DOI] (I p)