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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0631 [new locus tag: NWMN_RS03585 ]
  • pan locus tag?: SAUPAN002541000
  • symbol: vraG
  • pan gene symbol?: vraG
  • synonym:
  • product: ABC-type transport system permease component VraG

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0631 [new locus tag: NWMN_RS03585 ]
  • symbol: vraG
  • product: ABC-type transport system permease component VraG
  • replicon: chromosome
  • strand: +
  • coordinates: 711966..713867
  • length: 1902
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    GTGGCGTTAATTATGACCTTTAACGAGATAATATTTAAAAATTTCCGTCAAAATTTATCA
    CATTATGCCATCTATCTTTTTTCGTTAATTACGAGTGTAGTATTGTATTTTAGCTTTGTA
    GCATTAAAATACGCTCATAAACTAAACATGACAGAGTCATATCCAATTATAAAGGAAGGC
    TCACAAGTCGGAAGCTACTTTCTATTTTTCATCATAATTGCATTTTTGTTATATGCCAAT
    GTGTTATTTATTAAACGACGAAGTTATGAGCTTGCATTATATCAAACATTAGGTTTATCT
    AAATTCAACATTATTTATATACTAATGCTCGAACAATTACTAATATTTATAATTACGGCA
    ATATTAGGTATTATTATTGGTATTTTTGGTTCGAAACTGTTATTAATGATTGTCTTTACA
    TTATTAGGAATTAAAGAAAAGGTTCCAATTATTTTTAGTTTGAGGGCGGTATTTGAAACA
    TTAATGTTAATCGGTGTCGCTTATTTTTTAACATCTGCTCAAAATTTTATATTAGTGTTC
    AAACAATCTATTTCACAGATGTCAAAGAATAACCAGGTTAAAGAAACAAATCATAATAAA
    ATTACATTTGAAGAGGTTGTTTTAGGCATCTTAGGTATAGTATTGATTACCACAGGATAC
    TATCTATCTTTGAACATTGTTCAATATTATGATTCTATCGGTACACTTATGTTTATTTTA
    TTGTCAACTGTGATTGGGGCATACTTATTTTTTAAAAGCTCTGTTTCTCTAGTTTTTAAA
    ATGGTGAAGAAGTTTAGAAAAGGTGTTATAAGTGTAAATGATGTCATGTTCTCATCATCT
    ATTATGTATCGTATTAAGAAAAATGCTTTTTCACTTACGGTCATGGCAATCATTTCAGCG
    ATTACTGTTTCAGTTCTTTGCTTTGCTGCTATAAGTAGAGCGTCCTTATCAAGTGAAATA
    AAATATACTGCACCACACGACGTTACAATTAAAGACCAACAAAAAGCTAATCAATTAGCA
    AGTGAATTAAACAATCAAAAAATTCCTCATTTTTATAATTATAAAGAAGTAATTCATACG
    AAATTGTATAAAGATAATTTATTTGATGTAAAAGCGAAAGAACCATACAATGTAACAATT
    ACTAGTGATAAATACATCCCTAATACTGATTTGAAACGTGGGCAAGCTGATTTATTTGTA
    GCGGAAGGTTCTATCAAAGATTTAGTGAAACATAAGAAGCATGGTAAGGCAATTATAGGA
    ACGAAAAAACATCATGTTAATATTAAGTTACGTAAAGATATTAATAAAATCTATTTTATG
    ACAGATGTTGATTTAGGTGGACCAACGTTTGTCTTAAATGACAAAGACTATCAAGAAATA
    AGAAAGTATACAAAGGCAAAGCATATCGTCTCTCAATTTGGATTCGATTTGAAACATAAA
    AAAGATGCTTTAGCATTAGAAAAAGCGAAAAATAAAGTTGATAAATCTATTGAAACAAGA
    AGTGAAGCGATAAGCTCAATATCAAGTTTAACCGGAATATTATTATTTGTAACATCATTT
    TTAGGTATTACATTCTTGATTGCTGTATGTTGCATTATATACATAAAGCAAATAGATGAA
    ACCGAAGATGAGTTAGAGAATTATAGTATTTTGAGAAAGCTTGGATTTACACAAAAAGAT
    ATGGCAAGGGGACTAAAGTTTAAAATTATGTTTAATTTTGGGTTACCTTTAGTTATTGCA
    CTATCACATGCATATTTTACATCATTAGCATATATGAAATTAATGGGTACAACGAATCAA
    ATACCGGTTTTCATAGTAATGGGATTATACATTTGTATGTATGCTGTTTTTGCAGTGACG
    GCTTATAATCATTCCAAGCGAACAATTAGACATTCCATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1902

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0631 [new locus tag: NWMN_RS03585 ]
  • symbol: VraG
  • description: ABC-type transport system permease component VraG
  • length: 633
  • theoretical pI: 10.0671
  • theoretical MW: 72160.1
  • GRAVY: 0.371722

Function[edit | edit source]

  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 13.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 22.9)
    and 1 more
    no clan defined FxsA; FxsA cytoplasmic membrane protein (PF04186; HMM-score: 6.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00419
    • TAT(Tat/SPI): 0.000215
    • LIPO(Sec/SPII): 0.001519
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MALIMTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKLNMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIYILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTGILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]