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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0695 [new locus tag: NWMN_RS03930 ]
  • pan locus tag?: SAUPAN002621000
  • symbol: NWMN_0695
  • pan gene symbol?: bmrU
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0695 [new locus tag: NWMN_RS03930 ]
  • symbol: NWMN_0695
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 779043..779960
  • length: 918
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAAATAAATATACACATGGTGTCCTCTTTTACCATGAACACAGTGGATTAAAAAAT
    ATTAATCAAGGTATTGGGGAAGTTACAACAGCATTAAGTTCAATTTGTAAGCATCTTTCT
    ATTCAATTGAGTGAAAACGAAGGTGACATTATTAAATATTGCCAAGAAATCAAAACTAAA
    AACTATGCAAAAGATGTAGATATATTATTTATTCTTGGCGGTGATGGCACGGTTAACGAA
    CTTATAAACGGTGTTATGACACATGACCTTCAACTCCCTATTGGCATTTTACCAGGCGGT
    ACTTTTAATGATTTTACAAAAACGCTAAATATAGCACCTAATCATAAACAAGCTAGTGAA
    CAAATGATTTCTGCACAAGTTGGTACATACGATGTAATTAAAATAAATAATCAATATGCA
    CTCAACTTTGTTGGCTTAGGTCTCATTGTTCAAAACGCTGAAAACGTACAAGATGGTTCA
    AAAGATATATTTGGTAAATTGAGTTATATTGGATCAACTGTCAAAACACTGCTAAATCCA
    ACACAATTTAATTATCAATTATCAATAGATGATAAGACATATTCTGGCGAAACGACCATG
    ATACTAACAGCAAATGGTCCATTCATTGGTGGTAGTCGTATACCATTGACTGATTTGTCT
    CCTCAAGATGGTGAACTGAACACTTTCATTTTTAATGAACAAAGTTTCAGTATTTTAAAT
    GATATTTTTAAAAAGCGTGATAGCATGAATTGGAATGAAATTACTCAAGGTATTGAGCAT
    ATACCTGGTAAAAAGATTTCACTTACAACAGATCCAGCAATGAAAGTTGATATTGATGGC
    GAAATTTCATTAGAAACACCTATTGATATTGAGGTTATACCAAATGCCATTCAGTTACTT
    ACTGTCAATGATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0695 [new locus tag: NWMN_RS03930 ]
  • symbol: NWMN_0695
  • description: hypothetical protein
  • length: 305
  • theoretical pI: 4.49354
  • theoretical MW: 33625.9
  • GRAVY: -0.17082

Function[edit | edit source]

  • reaction:
    EC 2.7.1.-?  ExPASy
  • TIGRFAM:
    Unknown function Enzymes of unknown specificity lipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 168.7)
    and 1 more
    Unknown function Enzymes of unknown specificity lipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 76.9)
  • TheSEED:  
    Transcription regulator [contains diacylglycerol kinase catalytic domain]
  • PFAM:
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 93.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016346
    • TAT(Tat/SPI): 0.00054
    • LIPO(Sec/SPII): 0.002097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLSENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTFNDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGSKDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTDLSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKVDIDGEISLETPIDIEVIPNAIQLLTVNDL

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • NWMN_0695 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [1] [2]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]