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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
  • pan locus tag?: SAUPAN003206000
  • symbol: NWMN_0894
  • pan gene symbol?:
  • synonym:
  • product: 5' nucleotidase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
  • symbol: NWMN_0894
  • product: 5' nucleotidase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 993713..995227
  • length: 1515
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATGGAAAAAAATGAAAACATTAATGTAGAGATTTTAACTACGTCAGATATGCATAGT
    CATTTCTTAAATGGTGATTATGGTTCAAATATTTATAGAGCTGGTACTTATGTTAACCAA
    GTAAGAGCACAAAATCATCGCGTCATTTTATTAGATAGTGGCGGAAGTTTAGCTGGCTCG
    TTAGCGGCCTATTATTATGCTATTGTTGCACCTTATAAACGACATCCAATGATAAAGTTA
    ATGAACAGAATGCATTATGATGCTAGCGGTGTGAGTCCAAGTGAATTCAAGTTTGGTTTA
    TCATTTTTAACTCGTTCAATTGCTTTGGCACGTTTTCCATGGTTATCAGCAAATATTGAA
    TACAATGTTACTAAGGAGCCTTATTTTTCAACTCCATATTGTATTAAACATTTTGGTGAC
    TTAAAAATTGCTATCGTAGGCGTCACAGCAGATGGTTTAATGGAAAATGAGTATTCTGAA
    ATGGAGCAAGATGTATCTATTGAAAAGACATTAGTGGCATCAAAACGTTGGATTAGATAT
    ATCCATGAAGTTGAAGAGCCAGATTTTTTGATTGTAATTTATCATGGTGGATTGAATAAA
    ATTAGTAATAGTACGAAAAATAAAAAGGCAAGTTCGAATGAAGCTGAAAAATTAATGGAA
    GAACTCGGTGTTATAGATTTAATGATTACAGCTCATCAGCATCAAACAATAGTAGGTCAA
    GATCATGAAACGTATTATGTTCAGGCTGGTCAAGATGCCAAAGAGCTTGTACATCTTTCG
    ATTAATTTTAAAAAGAGAACAACAACTTATGATGTTGAAAGCATTGATTCTAAAGTGATT
    GACTTAAATGAGTATGAAGAGGATCAAGAATTATTAGATTTAACATTCTATGATAGAAAA
    GCAGTGGCTTATTGGTCACAGGAAATCATAAGTGATAAAGGTTTGATGTTATCAGTAAAT
    GGGTTACAAGATTTAGTCTGTCAAACACATCCATTTTCGCAATTATTACATGATGCAATT
    CACCTTGCATTTGATAATGATATAACATGTGTCCACGTGCCTATGAACGGAGAGAAGGGG
    TTGAGTGGACAGATTCGAAATGAAGATTTGTATCATGCATACCCATATCCAGATAAGCCA
    ATGGATATGACAATTAGTGGTCAAAATATCAAAGATATATTGGAGTATAGTTATTCACAT
    TTAGATTTTGTTAACGAGCAATTAAGCTTAACAATTATTGATGAAACGTTATGTACAATG
    TGGCAAGGATTCAATTATGAGATTGATATGAATCAAGAACCTGGGCAACGAGTAATGTTA
    GATCAAATTGATTTGACTAAGAGTTATAGAGTTACAATGACTGACTATTGTTATCGTAAC
    TACAAGAATTATTTAAAAAATGCTATTATACATGAATCATACGATGAAACAATGAGTACA
    TTAATTGCAGAGAAGTTAAGAGATCCGAATTATCGTATTTCATGTAGTGATAATTTTATA
    GTTAAAAACAGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1515

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
  • symbol: NWMN_0894
  • description: 5' nucleotidase family protein
  • length: 504
  • theoretical pI: 5.00359
  • theoretical MW: 58058.2
  • GRAVY: -0.397421

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 87.8)
    and 1 more
    Genetic information processing Protein synthesis Translation factors translation elongation factor Ts (TIGR00116; HMM-score: 14.6)
  • TheSEED:  
    Nucleosides and Nucleotides Purines Purine conversions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
    and 2 more
    Pyrimidines pyrimidine conversions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
    Potassium metabolism Potassium metabolism - no subcategory Glutathione-regulated potassium-efflux system and associated functions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
  • PFAM:
    no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 55.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.060077
    • TAT(Tat/SPI): 0.002964
    • LIPO(Sec/SPII): 0.004422
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • NWMN_0894 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]