From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
  • pan locus tag?: SAUPAN001421000
  • symbol: NWMN_0994
  • pan gene symbol?:
  • synonym:
  • product: phage cI-like repressor

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
  • symbol: NWMN_0994
  • product: phage cI-like repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1101519..1102238
  • length: 720
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGATAAAAAAGAATTAGCGAAATTTATAGGCAATAAAATCAGATACTATAGAACCAAA
    TTGAACTTAACTCAAGATCAACTTGGAGAAAAACTCAACACTAAAAAGGCTACTATTTCA
    AATTATGAGACAGGGTACAGAACTCCTAAACAAGATGATTTGTTTGAAATTGCTCATATT
    TTAAATATCAGTATCGATGATTTGTTTCCTACAAGAAATAATAAAAAAAACGACATCACT
    TCCATATACAGTAAACTCACGCCTCCAAGACAAAAAAACGTACTTAACTACGCAAATGAG
    CAATTAGATGAACAGAATAAAGTCACTTCTATAGATGAATATAAAGAGTCTAAACTAGTA
    TCGTATATTGCATGTGGTGCAACTGGTGCTGGCATAGGAGAAGAATTATATGATGACATA
    TTGCATGAAGAAGTATTTTTTAAAGAAGACGAAACGCCATCAAATGCTGATTTTTGTATT
    TTAGTTAATGGTGATTCAATGGAACCTATGTTAAAACAAGGAACATACGCTTTTATTAAG
    AAAGAAGATTCTATTAAAGATGGTACAATTGCACTCGTTGTATTAGATGGAGTAAGTCTT
    ATCAAGCGTGTAGATATATGCGAAGACTATATTAATTTGGTATCTCTAAATCCGAAGTAT
    GATGATATCAAAGTCGCTTCGTTTAGTAATATTAAAGTAATGGGCAAAGTTGTATTGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
  • symbol: NWMN_0994
  • description: phage cI-like repressor
  • length: 239
  • theoretical pI: 4.92692
  • theoretical MW: 27127.7
  • GRAVY: -0.442259

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 32.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 29)
    Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 29)
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 28.3)
    and 6 more
    Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 21.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glucose-1-phosphate adenylyltransferase, GlgD subunit (TIGR02092; EC 2.7.7.27; HMM-score: 19.8)
    signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 15.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 13.5)
    Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
    Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
  • TheSEED: data available for COL, NCTC8325
  • PFAM:
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 59.2)
    and 7 more
    Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 41.5)
    HTH (CL0123) HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 40.7)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 31.3)
    Pou; Pou domain - N-terminal to homeobox domain (PF00157; HMM-score: 16.1)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 14.7)
    no clan defined BNIP2; Bcl2-/adenovirus E1B nineteen kDa-interacting protein 2 (PF12496; HMM-score: 13.4)
    HTH (CL0123) MqsA_antitoxin; Antitoxin component of bacterial toxin-antitoxin system, MqsA (PF15731; HMM-score: 12.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005387
    • TAT(Tat/SPI): 0.000305
    • LIPO(Sec/SPII): 0.0006
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDKKELAKFIGNKIRYYRTKLNLTQDQLGEKLNTKKATISNYETGYRTPKQDDLFEIAHILNISIDDLFPTRNNKKNDITSIYSKLTPPRQKNVLNYANEQLDEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHEEVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLIKRVDICEDYINLVSLNPKYDDIKVASFSNIKVMGKVVL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]