PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1089 [new locus tag: NWMN_RS06175 ]
• pan locus tag^?: SAUPAN003449000
• symbol: mraW
• pan gene symbol^?: mraW
• synonym:
• product: S-adenosyl-methyltransferase MraW

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1089 [new locus tag: NWMN_RS06175 ]
• symbol: mraW
• product: S-adenosyl-methyltransferase MraW
• replicon: chromosome
• strand: +
• coordinates: 1192500..1193435
• length: 936
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330672 NCBI
• RefSeq: YP_001332123 NCBI
• BioCyc:
• MicrobesOnline: 3706641 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
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  661
  721
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  901
  
  GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAA
  AATGGTGTGTACATTGACTGTACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAAT
  CAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAGACCAAACTGCAATTGATAAT
  GCTAAAGAGGTATTAAAGGATCATTTGCATAGGGTGACTTTTGTTCATAGCAACTTCCGT
  GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGAC
  TTGGGTGTTTCAAGCCCACAACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGAC
  GCAACATTAGACATGCGTATGGACCAAACACAAGAACTAACAGCATATGAAATTGTTAAC
  AATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
  AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTA
  GAATTAGTTGACATTATAAAAGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACAT
  CCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAAACGATGAATTGTCAGCTTTT
  GAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGATGGCAGGATTTCGGTAATC
  ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGT
  CCAGAGGTACCAAGAGGATTACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGT
  GTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAGATGACAATAACAGAGCACGA
  AGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA
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  936
  

⊟Protein

⊟General

• locus tag: NWMN_1089 [new locus tag: NWMN_RS06175 ]
• symbol: MraW
• description: S-adenosyl-methyltransferase MraW
• length: 311
• theoretical pI: 5.97721
• theoretical MW: 35709.3
• GRAVY: -0.454984

⊟Function

• reaction:
  EC 2.1.1.199?  ExPASy
  16S rRNA (cytosine¹⁴⁰²-N⁴)-methyltransferase S-adenosyl-L-methionine + cytosine¹⁴⁰² in 16S rRNA = S-adenosyl-L-homocysteine + N⁴-methylcytosine¹⁴⁰² in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 409.6)
  and 1 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 14.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_5; MraW methylase family (PF01795; HMM-score: 456.7)
  and 9 more
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 31.9)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 22)
  rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 21.3)
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 18.5)
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 17.6)
  Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 16.7)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 14.6)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 14.5)
  GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003934
  • TAT(Tat/SPI): 0.000098
  • LIPO(Sec/SPII): 0.000233
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221301 NCBI
• RefSeq: YP_001332123 NCBI
• UniProt: A6QG79 UniProt

⊟Protein sequence

• MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1088 > mraW > ftsL > pbpA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications