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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1099 [new locus tag: NWMN_RS06225 ]
  • pan locus tag?: SAUPAN003460000
  • symbol: NWMN_1099
  • pan gene symbol?: sepF
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1099 [new locus tag: NWMN_RS06225 ]
  • symbol: NWMN_1099
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1204471..1205034
  • length: 564
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    GTGAGCCACTTGGCTTTAAAAGATTTATTTAGTGGATTTTTTGTAATAGATGATGAAGAG
    GAAGTAGAAGTACCTGACAAACAACAACAGGTAAATGAAGCGCCAGCAAAAGAGCAGTCA
    CAACAAACAACAAAACAAAACGCAATCAAATCAGTCCCTCAAAAATCTGCATCAAGATAT
    ACAACAACGTCAGAAGAAAGGAATAACCGTATGTCTAATTATTCAAAAAATAATTCACGT
    AATGTTGTAACTATGAACAATGCTACACCAAACAATGCATCACAAGAAAGTTCAAAAATG
    TGTTTATTCGAACCACGTGTTTTTTCAGATACACAAGATATTGCTGATGAGCTTAAAAAC
    CGCCGTGCGACACTTGTCAATTTACAACGTATTGATAAAGTATCAGCGAAAAGAATTATT
    GATTTTTTAAGCGGTACTGTTTATGCAATCGGTGGAGATATCCAACGTGTAGGTACTGAT
    ATTTTCTTATGTACGCCTGATAATGTGGAAGTAGCTGGAAGCATTACAGACCATATTGAA
    AATATGGAACATTCATTCGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    564

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1099 [new locus tag: NWMN_RS06225 ]
  • symbol: NWMN_1099
  • description: hypothetical protein
  • length: 187
  • theoretical pI: 4.78677
  • theoretical MW: 21023.1
  • GRAVY: -0.705348

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 11.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined SepF; Cell division protein SepF (PF04472; HMM-score: 98.1)
    and 4 more
    Ribosomal_L19e; Ribosomal protein L19e (PF01280; HMM-score: 13.6)
    PIN (CL0280) PIN_2; PIN domain (PF10130; HMM-score: 12.9)
    no clan defined SLC12; Solute carrier family 12 (PF03522; HMM-score: 12.3)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 4.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014794
    • TAT(Tat/SPI): 0.003857
    • LIPO(Sec/SPII): 0.002859
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTTKQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCLFEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTDIFLCTPDNVEVAGSITDHIENMEHSFD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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