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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1599 [new locus tag: NWMN_RS08965 ]
  • pan locus tag?: SAUPAN004354000
  • symbol: NWMN_1599
  • pan gene symbol?: —
  • synonym:
  • product: CBS domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1599 [new locus tag: NWMN_RS08965 ]
  • symbol: NWMN_1599
  • product: CBS domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1775877..1777175
  • length: 1299
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAAAACATGAACAAATTTTAGATTATATTGAATCGTTATCTATAGGCTCTAAAATT
    TCGGTGCGTAAAATCGCTAAATTTTTAAACGTATCTGAAGGGACAGCATATCGTGCAATT
    AAAGATGCTGATAAAATGGGCATGGTCGCAACGATAGATCGTGTTGGTACGGTTCGAATC
    GAAAAACGTAATCGTAATGAAATTGAACATTTAACTTTTAATGAAATTGTCAATATTATT
    GATGGCCAAGTGTTAGGTGGCAACAAAGGTATCACTAAGATGGTATCTAAATTTGCAATT
    GGAGCAATGGAACTCAAAGATATTTTAAAGTATATTGGTCCAAAGACATTATTGATTGTC
    GGAAATCGTGAAGATGTGCAGATTGAAGCTTTAAAAAGAGGTACGGCTATATTAATTACT
    GGTGGATTTAAGCCATCTAATAAAGTTATTAATTTTGCGAATGAACATGATTTACCTGTT
    CTATCATCAAGTTATGATACATTCCTAGTTGCAAATATTATCAACAAAGCACTTTTTAAT
    CAGAAAATACGAAAAGATATCTTAATAGTTCAAGATATAATGACGCCATTAGATGATTTA
    TCTGTGCTGTTTGATACGATGAAAATAGCAGATTATAAGCGTATGGCGAATCGAACTGGC
    CATACAAGATTTCCGGTAGTCAATGAGTCGTATAAATTAGTAGGTATTGTAACAAGTCGA
    GAGATGATTAATACAAAGGACGACGACGAAATAGATAAAGTGATGACGCGAAATCCTATT
    TATGTAAATGCTATGAGTACAGTAGCAAGTTGTGCGCATATGATGATTTGGGAAGGTATT
    GAATTGATTCCTGTCGTTTCGAGTAATAAAAAGACAGTGGGTGTGATTAATAGACAAGAT
    GTTTTAAAGTCTATGCAACTTTTAGGAAGACAGCCTCAGATGGGTGAAACAATTAACGAT
    CAAATCGCCAAATATATAACAATGAATCAAGATGGAATTACAGTAGAAGTTTCACCACTT
    TTAATCAATCATTACGGAACAGTAAGTAAAGCGGCATTTGTAAGTATTATAGAAGAAACA
    ATACAATATGAAATGCGTAAATTTAAAAAAGGTAATGTCATGATAGAGAACTTAAATATC
    GTGTATATTAAAACAGTACCTATTGAATCACATATAACAGTGCGCTTTGGCATACTAGAC
    GTCGGAAGAAACTTCGCCAAAATAGAAGTGAACATGCATAGTCAAAACGACAAAGTAGCG
    AGCGCTCTCGTGATATGCCAAATGTTTGACGAAGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1599 [new locus tag: NWMN_RS08965 ]
  • symbol: NWMN_1599
  • description: CBS domain-containing protein
  • length: 432
  • theoretical pI: 9.09376
  • theoretical MW: 48530.3
  • GRAVY: -0.0368056

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 42.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 37.2)
    and 5 more
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 25.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 21.6)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 19.4)
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 18.9)
    Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 12.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 102)
    and 17 more
    no clan defined CBS; CBS domain (PF00571; HMM-score: 49.3)
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 22.9)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 21.9)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.7)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 21.6)
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 19.6)
    no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 19.4)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 18.2)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15.5)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 14.9)
    HotDog (CL0050) 4HBT_2; Thioesterase-like superfamily (PF13279; HMM-score: 14.3)
    HTH (CL0123) HTH_22; HTH domain (PF13309; HMM-score: 14.3)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 13.6)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.8)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003311
    • TAT(Tat/SPI): 0.000513
    • LIPO(Sec/SPII): 0.000405
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDADKMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGAMELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPVLSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANRTGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMIWEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGITVEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHITVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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